在哺乳动物细胞中表达来自 的抗坏血酸过氧化物酶。
Expression of Ascorbate Peroxidase Derived from in Mammalian Cells.
机构信息
Division of Physiology, Kyushu Dental University, Fukuoka, Japan
Division of Molecular Signaling and Biochemistry, Kyushu Dental University, Kitakyushu, Fukuoka, Japan.
出版信息
In Vivo. 2020 Sep-Oct;34(5):2437-2441. doi: 10.21873/invivo.12058.
BACKGROUND/AIM: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity.
MATERIALS AND METHODS
The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, HO and acid stimulation.
RESULTS
Heat and HO stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, HO and acid stimulations than control cells lacking cAPX.
CONCLUSION
Introduction of cAPX increases the anti-oxidative capacity in mammalian cells.
背景/目的：来源于生活在高温和酸性环境中的原始红藻——Cyanidioschyzon merolae 的抗坏血酸过氧化物酶（APX）比其他植物中存在的类似过氧化物酶具有更强的抗氧化能力。在本研究中，我们研究了在哺乳动物细胞中表达来源于 Cyanidioschyzon merolae 的 APX（cAPX）是否会增加细胞的抗氧化能力。
材料和方法
将 cAPX 基因导入到小鼠成纤维样细胞系 C3H10T1/2 中。在经过热、HO 和酸刺激后，评估活性氧（ROS）的产生和/或细胞活力。
结果
热和 HO 刺激会导致 ROS 的产生。与缺乏 cAPX 的对照细胞相比，表达 cAPX 的细胞对热、HO 和酸刺激诱导的氧化应激更耐受。
结论
引入 cAPX 可增加哺乳动物细胞的抗氧化能力。
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