一种用于测定牛奶中游离羟基脂肪酸的液相色谱-高分辨率质谱（LC-HRMS）方法。
A Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) Method for the Determination of Free Hydroxy Fatty Acids in Cow and Goat Milk.
机构信息
Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.
出版信息
Molecules. 2020 Aug 29;25(17):3947. doi: 10.3390/molecules25173947.
A liquid chromatography-high resolution mass spectrometry (LC-HRMS) method for the direct determination of various saturated hydroxy fatty acids (HFAs) in milk was developed for the first time. The method involves mild sample preparation conditions, avoids time-consuming derivatization procedures, and permits the simultaneous determination of 19 free HFAs in a single 10-min run. This method was validated and applied in 17 cow milk and 12 goat milk samples. This work revealed the existence of various previously unrecognized hydroxylated positional isomers of palmitic acid and stearic acid in both cow and goat milk, expanding our knowledge on the lipidome of milk. The most abundant free HFAs in cow milk were proven to be 7-hydroxystearic acid (7HSA) and 10-hydroxystearic acid (10HSA) (mean content values of 175.1 ± 3.4 µg/mL and 72.4 ± 6.1 µg/mL in fresh milk, respectively). The contents of 7HSA in cow milk seem to be substantially higher than those in goat milk.
首次建立了一种用于直接测定乳中各种饱和羟基脂肪酸（HFAs）的液相色谱-高分辨质谱（LC-HRMS）方法。该方法具有温和的样品制备条件，避免了繁琐的衍生化步骤，可在 10 分钟的单次运行中同时测定 19 种游离 HFAs。对该方法进行了验证，并应用于 17 份牛乳和 12 份山羊乳样品中。该研究揭示了牛乳和山羊乳中存在各种以前未被识别的棕榈酸和硬脂酸的羟基化位置异构体，扩展了我们对乳脂组的认识。牛乳中最丰富的游离 HFAs 被证明为 7-羟基硬脂酸（7HSA）和 10-羟基硬脂酸（10HSA）（新鲜乳中的含量值分别为 175.1±3.4μg/mL 和 72.4±6.1μg/mL）。牛乳中 7HSA 的含量似乎明显高于山羊乳。
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