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利用Lowicryl免疫金技术在伯氏疟原虫蚊子阶段原位显示环子孢子蛋白的表达。

Expression of circumsporozoite proteins revealed in situ in the mosquito stages of Plasmodium berghei by the Lowicryl-immunogold technique.

作者信息

Hamilton A J, Davies C S, Sinden R E

机构信息

Department of Pure and Applied Biology, Imperial College, London.

出版信息

Parasitology. 1988 Apr;96 ( Pt 2):273-80. doi: 10.1017/s0031182000058273.

Abstract

A monoclonal antibody against the circumsporozoite proteins of the Plasmodium berghei sporozoite was used to trace the synthesis and expression of these proteins, via the Lowicryl immunogold technique, within the developing oocyst. The proteins were detected on the endoplasmic reticulum of the oocyst and were present in the sporozoite membranes at the point of their formation.

摘要

一种针对伯氏疟原虫子孢子环子孢子蛋白的单克隆抗体,通过Lowicryl免疫金技术,用于追踪这些蛋白在发育中的卵囊内的合成与表达。这些蛋白在卵囊的内质网上被检测到,并且在子孢子形成时存在于子孢子膜中。

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