Zhou Kai, Song Binbin, Wei Ming, Fang Jubo, Xu Yufen
Department of General Surgery, The Dongda Hospital, Shanxian, Heze, 274000 China.
Department of Oncology, The First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, 1882# Zhonghuan South Road, Jiaxing, 314000 China.
Cancer Cell Int. 2020 Aug 28;20:416. doi: 10.1186/s12935-020-01483-6. eCollection 2020.
This study aimed to investigate the relationship among miR-145-5p, and the NOD_LIKE_RECEPTOR pathway, thereby revealing the molecular mechanism of these three factors underlying the proliferation, migration and invasion of gastric cancer (GC) epithelial cells.
qRT-PCR was carried out to detect the expression of miR-145-5p and mRNA. Western blot was performed to test the protein levels of ANGPT2 as well as NOD1, NOD2 and NF-κB in the NOD_LIKE_RECEPTOR pathway. The targeting relationship between miR-145-5p and was verified via a dual-luciferase reporter gene assay. The proliferation, migration and invasion of GC cells were detected through MTT and Transwell assays, respectively.
The expression of miR-145-5p was significantly down-regulated in GC cells, while that of was notably up-regulated. MiR-145-5p directly bound with the 3'-UTR of mRNA, thereby targeting after transcription. Overexpression of miR-145-5p inhibited the proliferation, migration and invasion of GC cells by suppressing . Moreover, low expression of affected the protein levels of NOD1, NOD2 and NF-κB in the NOD_LIKE_RECEPTOR pathway, thus weakening the abilities of cell proliferation, migration and invasion.
MiR-145-5p plays an important role in GC epithelial cells, and it can affect cell proliferation, migration and invasion of GC cells by targeting and regulating the NOD_LIKE_RECEPTOR pathway. Overall, our study further elucidates the molecular mechanism underlying the malignant progression of GC.
本研究旨在探讨miR - 145 - 5p与NOD样受体途径之间的关系，从而揭示这三个因素影响胃癌（GC）上皮细胞增殖、迁移和侵袭的分子机制。
采用qRT - PCR检测miR - 145 - 5p和mRNA的表达。通过蛋白质免疫印迹法检测NOD样受体途径中ANGPT2以及NOD1、NOD2和NF - κB的蛋白水平。通过双荧光素酶报告基因检测验证miR - 145 - 5p与之间的靶向关系。分别通过MTT和Transwell实验检测GC细胞的增殖、迁移和侵袭能力。
miR - 145 - 5p在GC细胞中的表达显著下调，而的表达明显上调。miR - 145 - 5p直接与mRNA的3'-UTR结合，从而在转录后靶向。miR - 145 - 5p的过表达通过抑制来抑制GC细胞的增殖、迁移和侵袭。此外，的低表达影响了NOD样受体途径中NOD1、NOD2和NF - κB的蛋白水平，从而削弱了细胞增殖、迁移和侵袭的能力。
miR - 145 - 5p在GC上皮细胞中起重要作用，它可以通过靶向并调节NOD样受体途径来影响GC细胞的增殖、迁移和侵袭。总体而言，我们的研究进一步阐明了GC恶性进展的分子机制。
