Carraro Michela, Jones Kristen, Sartori Geppo, Schiavone Marco, Antonucci Salvatore, Kucharczyk Roza, di Rago Jean-Paul, Franchin Cinzia, Arrigoni Giorgio, Forte Michael, Bernardi Paolo
Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; Consiglio Nazionale delle Ricerche, Institute of Neuroscience, 35131 Padova, Italy.
Vollum Institute, OHSU, Portland, OR 97239, USA.
Cell Rep. 2020 Sep 1;32(9):108095. doi: 10.1016/j.celrep.2020.108095.
The mitochondrial permeability transition pore (PTP) is a Ca-activated channel that plays a key role in cell death. Thiol oxidation facilitates PTP opening, yet the targets and molecular mechanisms still await a definition. Here, we investigate the role of C141 of F-ATP synthase oligomycin sensitivity conferral protein (OSCP) subunit in PTP modulation by oxidation. We find that the OSCP C141S mutation confers resistance to PTP opening and cell death by diamide and MitoParaquat only when cyclophilin D (CyPD) has been ablated, a protective role that can be explained by CyPD shielding C141 from oxidants. The mutation decreases apoptosis in zebrafish embryos, indicating that this OSCP residue is involved in development. Site-directed mutagenesis in yeast suggests that other conserved cysteines in the α, γ, and c subunits of F-ATP synthase are not involved in PTP modulation. Thus, OSCP provides a strategic site that regulates PTP opening by the interplay between CyPD (un)binding and thiol oxidation-reduction.
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