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宏分类组学有助于揭示巴西乳制品中的微生物群。

Metataxonomics contributes to unravel the microbiota of a Brazilian dairy.

机构信息

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.

Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, São Paulo, Brazil.

出版信息

J Dairy Res. 2020 Aug;87(3):360-363. doi: 10.1017/S0022029920000837. Epub 2020 Sep 4.

Abstract

For this research communication, 90 samples of a Brazilian dairy were combined into four groups (raw material, final product, food-contact and non-food contact surfaces) and analyzed by metataxonomics based on 16S rRNA gene sequencing. The results showed high alpha-diversity indexes for final product and non-food contact surfaces but, overall, beta-diversity indexes were low. The samples were separated in two main clusters, and the core microbiota was composed by Macrococcus, Alkaliphilus, Vagococcus, Lactobacillus, Marinilactibacillus, Streptococcus, Lysinibacillus, Staphylococcus, Clostridium, Halomonas, Lactococcus, Enterococcus, Bacillus and Psychrobacter. These results highlight that rare taxa occur in dairies, and this may aid the development of strategies for food protection.

摘要

本研究通讯将巴西乳制品的 90 个样本组合成四个组(原料、最终产品、食品接触面和非食品接触面),并通过基于 16S rRNA 基因测序的分类组学进行分析。结果表明,最终产品和非食品接触面的 alpha 多样性指数较高,但总体而言,beta 多样性指数较低。样品分为两个主要聚类,核心微生物群由巨球菌属、嗜堿菌属、乳球菌属、乳杆菌属、海洋乳杆菌属、链球菌属、赖氨酸芽孢杆菌属、葡萄球菌属、梭菌属、盐单胞菌属、乳球菌属、肠球菌属、芽孢杆菌属和嗜冷杆菌属组成。这些结果表明,稀有分类群在乳制品中存在,这可能有助于制定食品保护策略。

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