Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Indian J Med Microbiol. 2020 Apr-Jun;38(2):162-168. doi: 10.4103/ijmm.IJMM_20_153.
Methicillin-resistant Staphylococcus aureus (MRSA) has become more prevalent all over the world and it is important to determine MRSA prevalence and typing in different regions. The present study was carried out to determine the prevalence and frequency of circulating molecular types of MRSA isolates as well as their antibiotics susceptibility in Tabriz and Kerman cities of Iran.
A total of 230 S. aureus isolates were collected from Tabriz (n=125) and Kerman (n=105) during January to December 2018. MRSA isolates were identified by PCR amplification of nuc and mec A genes. Antibiotic susceptibility of MRSA isolates were determined by Kirby-Bauer disk diffusion method. Multiplex PCR was exploited to detect various types of SCCmec.
The MRSA prevalence was 51/125 (40.8%) in Tabriz and 60/105 (57.1%) in Kerman. Overall, 36/51 (70.58%) and 15/51 (29.41%) isolates and 37/60 (61.66%) and 23/60 (38.34%) isolates were isolated from inpatients and outpatients in Tabriz and Kerman, respectively. Almost all of the isolates were resistant to penicillin and all of them were sensitive to linezolid. Thirty five (68.2%) and 34(56.6%) of MRSA isolates in Tabriz and Kerman were determined as MDR, respectively. SCCmec typing showed that the frequent SCCmec type in both Tabriz and Kerman cities was SCCmec III (56.86% and 55%, respectively).
The high prevalence of MRSA makes it necessary to revisit the antibiotics administration by physicians. Indeed, periodic evaluation of antibacterial susceptibility patterns of the MRSA strains is required for efficient treatment of MRSA infections.
耐甲氧西林金黄色葡萄球菌（MRSA）在世界各地的流行率越来越高，因此确定不同地区的 MRSA 流行率和分型非常重要。本研究旨在确定伊朗大不里士和克尔曼市 MRSA 分离株的流行率和循环分子型的频率以及它们对抗生素的敏感性。
2018 年 1 月至 12 月，从大不里士（n=125）和克尔曼（n=105）收集了 230 株金黄色葡萄球菌分离株。通过聚合酶链反应（PCR）扩增 nuc 和 mecA 基因鉴定 MRSA 分离株。采用 Kirby-Bauer 纸片扩散法测定 MRSA 分离株的抗生素敏感性。采用多重 PCR 检测各种类型的 SCCmec。
大不里士的 MRSA 流行率为 51/125（40.8%），克尔曼为 60/105（57.1%）。总的来说，36/51（70.58%）和 15/51（29.41%）株和 37/60（61.66%）和 23/60（38.34%）株分别从大不里士和克尔曼的住院患者和门诊患者中分离出来。几乎所有的分离株对青霉素均耐药，对利奈唑胺均敏感。大不里士和克尔曼的 35（68.2%）和 34（56.6%）株 MRSA 被确定为 MDR。SCCmec 分型显示，大不里士和克尔曼两城市常见的 SCCmec 型均为 SCCmec III（分别为 56.86%和 55%）。
MRSA 的高流行率使得医生有必要重新审视抗生素的使用。事实上，需要定期评估 MRSA 菌株的抗菌药敏模式，以有效治疗 MRSA 感染。
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