过氧化氢作为一种氧化剂，用于使用 Schiff 试剂在酒精和水固定剂固定的兔肝中显示糖原。

Hydrogen peroxide as an oxidizing agent for glycogen demonstration using Schiff's reagent in rabbit liver fixed with alcoholic and aqueous fixatives.

机构信息

Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.

Department of Pathology, College of Medicine, University of Hail, Hail, Kingdom of Saudi Arabia.

出版信息

J Histotechnol. 2021 Mar;44(1):20-26. doi: 10.1080/01478885.2020.1816010. Epub 2020 Sep 10.

DOI:10.1080/01478885.2020.1816010

PMID:32909929

Abstract

This study investigated the optimal concentration of hydrogen peroxide (HO) for demonstrating glycogen in rabbit liver using Schiff's reaction as compared to the conventional periodic acid-Schiff (PAS) method. Four fixatives, Bouin's, neutral buffered formalin (NBF), 80% ethanol, and Rossman's, were used at room temperature (RT) and 4°C. Paraffin sections of rabbit liver (n = 192) were stained using 0.5%, 1% and 2% periodic acid-Schiff (PAS) and 0.5%, 1%, and 2% hydrogen peroxide (HO)-Schiff methods. It was found that 0.5% HO-Schiff provided good staining results, while 1% PAS produced excellent results for glycogen fixed with 80% ethanol at RT. Also, 0.5% HO-Schiff produced good staining of glycogen fixed with 10% NBF at RT as compared to 0.5% PAS which provided satisfactory staining results. Fixation with 80% ethanol at RT produced the best staining quality for glycogen using both oxidizing agents at the optimal concentrations of either 1% periodic acid or 0.5% HO.

摘要

本研究通过 Schiff 反应比较了不同浓度的过氧化氢（HO）在显示兔肝糖原中的最佳浓度，与传统的过碘酸希夫（PAS）方法相比。本研究使用了 4 种固定剂，即 Bouin's 液、中性缓冲福尔马林（NBF）、80%乙醇和 Rossman's 液，在室温（RT）和 4°C 下进行固定。使用 0.5%、1%和 2%的 PAS 和 0.5%、1%和 2%的 HO-Schiff 方法对兔肝石蜡切片进行染色。结果发现，0.5% HO-Schiff 提供了良好的染色结果，而 1% PAS 对 RT 下 80%乙醇固定的糖原产生了极好的结果。此外，与 0.5% PAS 相比，0.5% HO-Schiff 对 RT 下 10% NBF 固定的糖原具有良好的染色效果。在 RT 下，80%乙醇固定对两种最佳浓度的氧化剂（1%过碘酸或 0.5% HO）均能产生最佳的糖原染色质量。