Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.
Department of Pathology, College of Medicine, University of Hail, Hail, Kingdom of Saudi Arabia.
J Histotechnol. 2021 Mar;44(1):20-26. doi: 10.1080/01478885.2020.1816010. Epub 2020 Sep 10.
This study investigated the optimal concentration of hydrogen peroxide (HO) for demonstrating glycogen in rabbit liver using Schiff's reaction as compared to the conventional periodic acid-Schiff (PAS) method. Four fixatives, Bouin's, neutral buffered formalin (NBF), 80% ethanol, and Rossman's, were used at room temperature (RT) and 4°C. Paraffin sections of rabbit liver (n = 192) were stained using 0.5%, 1% and 2% periodic acid-Schiff (PAS) and 0.5%, 1%, and 2% hydrogen peroxide (HO)-Schiff methods. It was found that 0.5% HO-Schiff provided good staining results, while 1% PAS produced excellent results for glycogen fixed with 80% ethanol at RT. Also, 0.5% HO-Schiff produced good staining of glycogen fixed with 10% NBF at RT as compared to 0.5% PAS which provided satisfactory staining results. Fixation with 80% ethanol at RT produced the best staining quality for glycogen using both oxidizing agents at the optimal concentrations of either 1% periodic acid or 0.5% HO.
本研究通过 Schiff 反应比较了不同浓度的过氧化氢（HO）在显示兔肝糖原中的最佳浓度，与传统的过碘酸希夫（PAS）方法相比。本研究使用了 4 种固定剂，即 Bouin's 液、中性缓冲福尔马林（NBF）、80%乙醇和 Rossman's 液，在室温（RT）和 4°C 下进行固定。使用 0.5%、1%和 2%的 PAS 和 0.5%、1%和 2%的 HO-Schiff 方法对兔肝石蜡切片进行染色。结果发现，0.5% HO-Schiff 提供了良好的染色结果，而 1% PAS 对 RT 下 80%乙醇固定的糖原产生了极好的结果。此外，与 0.5% PAS 相比，0.5% HO-Schiff 对 RT 下 10% NBF 固定的糖原具有良好的染色效果。在 RT 下，80%乙醇固定对两种最佳浓度的氧化剂（1%过碘酸或 0.5% HO）均能产生最佳的糖原染色质量。
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