基于抗生素和哺乳动物 IgG 的侧向流动分析检测金黄色葡萄球菌的简单而灵敏的方法。
Antibiotic and mammal IgG based lateral flow assay for simple and sensitive detection of Staphylococcus aureus.
机构信息
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China.
出版信息
Food Chem. 2021 Mar 1;339:127955. doi: 10.1016/j.foodchem.2020.127955. Epub 2020 Sep 2.
Lateral flow assay (LFA), performed with simple devices and short detection time, is popular in field applications. Herein, a novel sandwich type-based LFA was constructed for high sensitivity and selectivity detection of Staphylococcus aureus (S. aureus). Vancomycin-immobilized gold nanoparticles (VAN-Au NPs) were utilized as the first identifier to capture S. aureus and the specificity was guaranteed by the second recognition agent of pig immunoglobulin G (IgG). In addition, gold growth was adopted for signal amplification to further improve the detection sensitivity. S. aureus could be directly assayed by this LFA within the concentration range of 1.0 × 10-1.0 × 10 cfu mL with a detection limit of 1.0 × 10 cfu mL. Furthermore, the novel sandwich LFA realized S. aureus detection in food samples with admissible recoveries and established a rapid, simple, cost-effective and sensitive platform, could meet the demand for on-site testing of S. aureus.
侧向流动检测（LFA），采用简单的设备和短的检测时间，在现场应用中很受欢迎。本文构建了一种新型基于三明治结构的侧向流动检测法，用于高灵敏度和选择性检测金黄色葡萄球菌（S. aureus）。万古霉素固定化金纳米颗粒（VAN-Au NPs）被用作第一个识别体来捕获 S. aureus，而第二个识别剂为猪免疫球蛋白 G（IgG），以保证特异性。此外，采用金增长进行信号放大，以进一步提高检测灵敏度。通过该侧向流动检测法，可以直接检测浓度范围为 1.0×10-1.0×10cfu mL 内的 S. aureus，检测限为 1.0×10cfu mL。此外，新型三明治侧向流动检测法可实现食品样本中金黄色葡萄球菌的检测，回收率可接受，建立了一个快速、简单、经济高效且敏感的平台，可满足现场检测金黄色葡萄球菌的需求。
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