Jaafar Nurjamalina Fasihah, Ramli Muhammad Ezzudin, Mohd Salleh Rabeta
Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 USM Pulau Pinang, Malaysia.
Trop Life Sci Res. 2020 Jul;31(2):1-17. doi: 10.21315/tlsr2020.31.2.1. Epub 2020 Aug 6.
is a herbaceous plant with many health benefits. Extraction is crucial to obtain its bioactive components which contribute to its antioxidant properties. Therefore, this study was conducted to find an optimum extraction condition of flower on total phenolic content (TPC) and antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging activity) as well as to determine its total flavonoid content (TFC) and anthocyanin content based on the optimum extraction condition generated by Response Surface Methodology (RSM)-Design Expert 7.1.5. TPC, TFC and total anthocyanin of were conducted by Folin Ciocalteu (FC), calorimetric assay and pH differential method, respectively. The ranges of selected independent variables were ethanol concentration (30°C-90% v/v), time (60-120 min) and temperature (30°C-70°C). The optimum extraction condition was obtained at 39.62% v/v ethanol concentration, 90 min and 44.24°C. However, these values were slightly adjusted according to the convenience of equipment to operate in which ethanol concentration was adjusted to 37% v/v, time remain at 90 min and temperature at 45°C. The predicted values of TPC and DPPH radical scavenging activity were 41.60 mg GAE/g dry samples and 68.12% inhibition and were experimentally verified to be 41.17 ± 0.5 mg GAE/g dry samples and 63.53 ± 0.95% inhibition of TPC and DPPH radical scavenging activity respectively. This result has showed RSM can optimise TPC and radical scavenging activity of . Upon the optimum condition, the TFC determined was 187.05 ± 3.18 mg quercetin/g dried sample which was higher than TPC and the total anthocyanin content was 28.60 ± 0.04 mg/L. Hence, the extractable phenolic, flavonoid and anthocyanin compounds indicated that is a good source of natural antioxidant.
是一种具有多种健康益处的草本植物。提取对于获得其生物活性成分至关重要，这些成分有助于其抗氧化特性。因此，本研究旨在寻找花的最佳提取条件，以确定其总酚含量（TPC）和抗氧化活性（2,2-二苯基-1-苦基肼（DPPH）自由基清除活性），并基于响应面法（RSM）-Design Expert 7.1.5生成的最佳提取条件确定其总黄酮含量（TFC）和花青素含量。分别采用福林-酚（FC）法、比色法和pH差值法测定的TPC、TFC和总花青素。所选自变量的范围为乙醇浓度（30%-90% v/v）、时间（60-120分钟）和温度（30°C-70°C）。在乙醇浓度为39.62% v/v、时间为90分钟和温度为44.24°C时获得了最佳提取条件。然而，根据设备操作的便利性对这些值进行了微调，其中乙醇浓度调整为37% v/v，时间保持在90分钟，温度为45°C。TPC和DPPH自由基清除活性的预测值分别为41.60 mg GAE/g干样品和68.12%抑制率，经实验验证，TPC和DPPH自由基清除活性分别为41.17±0.5 mg GAE/g干样品和63.53±0.95%抑制率。该结果表明RSM可以优化的TPC和自由基清除活性。在最佳条件下，测定的TFC为187.05±3.18 mg槲皮素/g干样品，高于TPC，总花青素含量为28.60±0.04 mg/L。因此，可提取的酚类、黄酮类和花青素化合物表明是天然抗氧化剂的良好来源。
