Cooney M, Czernuszewicz G, Postel E H, Flint S J, Hogan M E
Department of Molecular Biology, Princeton University, NJ 08544.
Science. 1988 Jul 22;241(4864):456-9. doi: 10.1126/science.3293213.
A 27-base-long DNA oligonucleotide was designed that binds to duplex DNA at a single site within the 5' end of the human c-myc gene, 115 base pairs upstream from the transcription origin P1. On the basis of the physical properties of its bound complex, it was concluded that the oligonucleotide forms a colinear triplex with the duplex binding site. By means of an in vitro assay system, it was possible to show a correlation between triplex formation at -115 base pairs and repression of c-myc transcription. The possibility is discussed that triplex formation (site-specific RNA binding to a DNA duplex) could serve as the basis for an alternative program of gene control in vivo.
设计了一段27个碱基长的DNA寡核苷酸，它能在人c-myc基因5'端的一个位点与双链DNA结合，该位点位于转录起始点P1上游115个碱基对处。根据其结合复合物的物理性质，得出该寡核苷酸与双链结合位点形成共线三链体的结论。通过体外检测系统，有可能显示在-115个碱基对处形成三链体与c-myc转录抑制之间的相关性。文中讨论了三链体形成（位点特异性RNA与DNA双链结合）可能作为体内基因控制另一种程序基础的可能性。
