一组鼠李糖基化特异性抗体能够检测细菌中新型蛋白质糖基化。
A set of rhamnosylation-specific antibodies enables detection of novel protein glycosylations in bacteria.
作者信息
Gast Daniel, Koller Franziska, Krafczyk Ralph, Bauer Lukas, Wunder Swetlana, Lassak Jürgen, Hoffmann-Röder Anja
机构信息
Department of Chemistry, Ludwig-Maximilians-Universität München, Munich, Germany.
出版信息
Org Biomol Chem. 2020 Sep 21;18(35):6823-6828. doi: 10.1039/d0ob01289k. Epub 2020 Sep 2.
Despite its potential importance for bacterial virulence, protein rhamnosylation has not yet been sufficiently studied. Specific anti-Ser, anti-Thr and anti-Asn antibodies allowed the identification of previously unknown monorhamnosylated proteins in cytosol and membrane fractions of bacterial cell lysates. Mapping of the complete rhamnoproteome in pathogens should facilitate development of targeted therapies against bacterial infections.
尽管蛋白质鼠李糖基化对细菌毒力具有潜在重要性，但尚未得到充分研究。特异性抗丝氨酸、抗苏氨酸和抗天冬酰胺抗体能够鉴定细菌细胞裂解物的胞质溶胶和膜组分中先前未知的单鼠李糖基化蛋白。绘制病原体中完整的鼠李糖蛋白质组图谱应有助于开发针对细菌感染的靶向疗法。
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