7B2蛋白在人、猪和大鼠甲状腺以及人髓样癌中的鉴定与定位。

Identification and localization of 7B2 protein in human, porcine, and rat thyroid gland and in human medullary carcinoma.

作者信息

Marcinkiewicz M, Benjannet S, Falgueyret J P, Seidah N G, Schürch W, Verdy M, Cantin M, Chrétien M

机构信息

J. A. de Sève Laboratories of Biochemical and Molecular Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada.

出版信息

Endocrinology. 1988 Aug;123(2):866-73. doi: 10.1210/endo-123-2-866.

DOI:10.1210/endo-123-2-866

PMID:3293987

Abstract

The novel, highly conserved polypeptide 7B2, which belongs to a new protein superfamily, was isolated from human and porcine hypophysis. The availability of a specific antibody to a synthetic fragment enabled 7B2 localization in a number of neurocrine and endocrine tissues and revealed its secretory character. 7B2 was purified from thyroid homogenates by HPLC chromatography and characterized by gel permeation chromatography (dimeric mol wt, approximately 40,000) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (monomeric mol wt, 20,750). By immunocytochemistry 7B2 was colocalized with calcitonin in parafollicular cells and identified within secretory granules by electron microscopy. Three of nineteen human medullary carcinoma cases showed immunoreactive 7B2 within the early and late hyperplasia stages and neoplasia. Results suggest that 7B2 may play a role in endocrine function, possibly as a secretory substance, and may be a histochemical marker in addition to calcitonin for medullary carcinoma.

摘要

新型高度保守多肽7B2属于一个新的蛋白质超家族，它是从人和猪的垂体中分离出来的。针对合成片段的特异性抗体的可用性使得7B2能够在许多神经分泌和内分泌组织中定位，并揭示了其分泌特性。7B2通过高效液相色谱法从甲状腺匀浆中纯化出来，并通过凝胶渗透色谱法（二聚体分子量约为40,000）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法（单体分子量为20,750）进行表征。通过免疫细胞化学方法，7B2与降钙素在滤泡旁细胞中共定位，并通过电子显微镜在分泌颗粒中得以鉴定。在19例人类髓样癌病例中，有3例在早期和晚期增生阶段以及肿瘤形成过程中显示出7B2免疫反应性。结果表明，7B2可能在内分泌功能中发挥作用，可能作为一种分泌物质，并且除了降钙素之外，可能还是髓样癌的一种组织化学标志物。