INSERM U963, CNRS UPR9022, University of Strasbourg, Strasbourg, France.
SERPICO Inria Team/CNRS UMR 144, Institut Curie, Paris, France.
PLoS Pathog. 2020 Sep 18;16(9):e1008739. doi: 10.1371/journal.ppat.1008739. eCollection 2020 Sep.
Malaria-causing Plasmodium parasites traverse the mosquito midgut cells to establish infection at the basal side of the midgut. This dynamic process is a determinant of mosquito vector competence, yet the kinetics of the parasite migration is not well understood. Here we used transgenic mosquitoes of two Anopheles species and a Plasmodium berghei fluorescence reporter line to track parasite passage through the mosquito tissues at high spatial resolution. We provide new quantitative insight into malaria parasite invasion in African and Indian Anopheles species and propose that the mosquito complement-like system contributes to the species-specific dynamics of Plasmodium invasion.
疟原虫通过蚊子的中肠细胞迁移到中肠基底侧建立感染。这一动态过程是蚊媒传播能力的决定因素，但寄生虫迁移的动力学尚未完全清楚。本研究利用两种按蚊的转基因蚊和伯氏疟原虫荧光报告株，以高空间分辨率追踪寄生虫在蚊组织中的穿透过程。本研究为了解非洲和印度按蚊物种中的疟原虫入侵提供了新的定量见解，并提出蚊子补体样系统有助于疟原虫入侵的种间特异性动态。
