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防腐尸体组织的组织学可靠性:它们在医学教育中有用吗?

Histologic Reliability of Tissues from Embalmed Cadavers: Can They be Useful in Medical Education?

作者信息

Abuhaimed Abdulaziz K, Almulhim Abdulaziz M, Alarfaj Faris A, Almustafa Salam S, Alkhater Khulood M, Al Yousef Mohammed J, Al Bayat Methal I, Madadin Mohammed, Menezes Ritesh G

机构信息

College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

出版信息

Saudi J Med Med Sci. 2020 Sep-Dec;8(3):208-212. doi: 10.4103/sjmms.sjmms_383_19. Epub 2020 Aug 20.

Abstract

BACKGROUND

Current trends in medical curricula are shifting from teaching histology and pathology as stand-alone disciplines. Therefore, it would be useful to examine the potential value of integrating these into the anatomical dissection experience.

OBJECTIVES

The aim of this study was to assess the histologic reliability of tissues taken from embalmed cadavers in an anatomy laboratory.

MATERIALS AND METHODS

A total of 112 tissue samples were obtained using standard autopsy techniques from various organs (heart, lung, thyroid, skeletal muscle, bone and skin) of 11 cadavers available at the anatomy laboratory of Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia, in 2019. Samples were prepared using the standard paraffin procedure followed by cutting sections at 4-μm thickness and staining with standard hematoxylin and eosin stain. Using predefined criteria, the quality of the samples was evaluated by two board-certified histopathologists and each slide was categorized as good, satisfactory or poor.

RESULTS

Overall, 34.2% and 60.3% of the slides were of good and satisfactory quality, respectively. A significant difference in tissue quality was found between various organs. Thick skin and bone tissues had the highest "good" rating (84.6% and 81.8%, respectively), while thyroid and lung tissues had the highest "poor" rating (20% and 13.6%, respectively).

CONCLUSION

Most of the tissues acquired from the embalmed cadavers were of good or satisfactory quality, thereby indicating the beneficial use of histological tissue from cadavers for educational purposes. Future research into how these findings translate into meaningful medical education would be beneficial.

摘要

背景

医学课程的当前趋势正从将组织学和病理学作为独立学科进行教学转变。因此,研究将这些学科整合到解剖学解剖体验中的潜在价值将是有益的。

目的

本研究的目的是评估解剖实验室中从防腐尸体获取的组织的组织学可靠性。

材料和方法

2019年,在沙特阿拉伯达曼伊玛目阿卜杜勒拉赫曼·本·费萨尔大学的解剖实验室,使用标准尸检技术从11具尸体的各种器官(心脏、肺、甲状腺、骨骼肌、骨骼和皮肤)中总共获取了112个组织样本。样本采用标准石蜡程序制备,然后切成4μm厚的切片,并用标准苏木精和伊红染色。根据预先确定的标准,由两名获得委员会认证的组织病理学家对样本质量进行评估,每张载玻片分为良好、满意或差三类。

结果

总体而言,分别有34.2%和60.3%的载玻片质量良好和令人满意。不同器官之间的组织质量存在显著差异。厚皮肤和骨骼组织的“良好”评级最高(分别为84.6%和81.8%),而甲状腺和肺组织的“差”评级最高(分别为20%和13.6%)。

结论

从防腐尸体获取的大多数组织质量良好或令人满意,从而表明尸体的组织学组织在教育目的方面的有益用途。对这些发现如何转化为有意义的医学教育进行未来研究将是有益的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f202/7485655/7efcc834f01c/SJMMS-8-208-g001.jpg

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