Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
Hefei Cancer Hospital, Chinese Academy of Sciences, 350 Shushanhu Road, Hefei, 230031, Anhui, China.
Biotechnol Lett. 2021 Jan;43(1):25-34. doi: 10.1007/s10529-020-03007-8. Epub 2020 Sep 21.
The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood.
After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be detected while less than 10% of the same cells could be detected by flow cytometry (FCM) if spiked into blood. However, in combination with CD326 and CD45 immunomagnetic beads, detection rate of MCF7 cells in blood reached 57%. For circulating tumor cells, enrichment by CD326 and CD45 immunomagnetic beads improve the detection rate from nearly undetectable to more than 24.14%.
Live CTCs in peripheral blood can be effectively and sensitively detected by using a combination of immunomagnetic beads (CD45 and CD326) and flow cytometry.
本文旨在建立一种使用免疫磁珠和流式细胞术快速富集和检测外周血循环肿瘤细胞（CTC）的方法。
用 CD326-PE 和 CD45-APC 抗体孵育后，在 M-Buffer 中可检测到超过 60%的 MCF7 细胞，而如果将相同数量的 MCF7 细胞掺入血液中，则通过流式细胞术（FCM）检测不到 10%。然而，与 CD326 和 CD45 免疫磁珠联合使用时，血液中 MCF7 细胞的检测率达到 57%。对于循环肿瘤细胞，CD326 和 CD45 免疫磁珠的富集可将检测率从几乎无法检测提高到 24.14%以上。
使用免疫磁珠（CD45 和 CD326）和流式细胞术可有效、灵敏地检测外周血中的活 CTC。
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