Miran Isabelle, Scherer Dominique, Ostyn Pauline, Mazouni Chafika, Drusch Françoise, Bernard Marine, Louvet Emilie, Adam Julien, Mathieu Marie-Christine, Haffa Mariam, Antignac Jean-Philippe, Le Bizec Bruno, Vielh Philippe, Dessen Philippe, Perdry Hervé, Delaloge Suzette, Feunteun Jean
Translational Research Lab, INSERM U981, Université Paris-Saclay, Villejuif, France.
Division of Preventive Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ), Heidelberg, Germany.
Front Oncol. 2020 Aug 21;10:1506. doi: 10.3389/fonc.2020.01506. eCollection 2020.
The tissue stroma plays a major role in tumors' natural history. Most programs for tumor progression are not activated as cell-autonomous processes but under the conditions of cross-talks between tumor and stroma. Adipose tissue is a major component of breast stroma. This study compares adipose tissues in tumor-bearing breasts to those in tumor-free breasts with the intention of defining a signature that could translate into markers of cancer risk. In tumor-bearing breasts, we sampled adipose tissues adjacent to, or distant from the tumor. Parameters studied included: adipocytes size and density, immune cell infiltration, vascularization, secretome and gene expression. Adipose tissues from tumor-bearing breasts, whether adjacent to or distant from the tumor, do not differ from each other by any of these parameters. By contrast, adipose tissues from tumor-bearing breasts have the capacity to secrete twice as much interleukin 8 (IL-8) than those from tumor-free breasts and differentially express a set of 137 genes of which a significant fraction belongs to inflammation, integrin and wnt signaling pathways. These observations show that adipose tissues from tumor-bearing breasts have a distinct physiological status from those from tumor-free breasts. We propose that this constitutive status contributes as a non-cell autonomous process to determine permissiveness for tumor growth.
组织基质在肿瘤的自然病程中起主要作用。大多数肿瘤进展程序并非作为细胞自主过程被激活，而是在肿瘤与基质相互作用的条件下被激活。脂肪组织是乳腺基质的主要成分。本研究比较了荷瘤乳腺中的脂肪组织与无瘤乳腺中的脂肪组织，旨在确定一种可转化为癌症风险标志物的特征。在荷瘤乳腺中，我们采集了肿瘤附近或远处的脂肪组织。研究的参数包括：脂肪细胞大小和密度、免疫细胞浸润、血管生成、分泌组和基因表达。荷瘤乳腺中的脂肪组织，无论与肿瘤相邻还是远离肿瘤，在这些参数上彼此并无差异。相比之下，荷瘤乳腺中的脂肪组织分泌白细胞介素8（IL-8）的能力是无瘤乳腺中脂肪组织的两倍，并且差异表达一组137个基因，其中很大一部分属于炎症、整合素和Wnt信号通路。这些观察结果表明，荷瘤乳腺中的脂肪组织与无瘤乳腺中的脂肪组织具有不同的生理状态。我们提出，这种固有状态作为一种非细胞自主过程，有助于确定肿瘤生长的允许性。
