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对大肠杆菌O111:B4脂多糖(LPS)的外核心多糖和O抗原具有特异性的免疫保护性鼠单克隆抗体。

Immunoprotective murine monoclonal antibodies specific for the outer-core polysaccharide and for the O-antigen of Escherichia coli 0111:B4 lipopolysaccharide (LPS).

作者信息

Coughlin R T, Bogard W C

出版信息

J Immunol. 1987 Jul 15;139(2):557-61.

PMID:3298431
Abstract

The antigen specificity of two immunoprotective monoclonal antibodies derived from mice immunized with Escherichia coli 0111:B4 bacteria and boosted with purified lipopolysaccharide (LPS) were investigated. One of the antibodies, B7, was shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunostaining to bind to the O-antigen containing LPS species, whereas the other antibody, 5B10, reacted with both O-antigen containing homologs and the O-antigen-deficient LPS. 5B10 did not bind to LPS from E. coli J5, an Rc mutant of E. coli 0111:B4 that lacks both the O-antigen and outer core sugars. 5B10 did not cross-react with LPS from several other E. coli strains. Thus 5B10 appeared to recognize a type-specific epitope in the outer core of LPS exclusive of Rc determinants. The monoclonal antibody specific for the polymeric O-antigen is of the IgG3 subclass, and the monoclonal antibody 5B10 specific for the outer core of LPS is an IgG2a. Although B7 and 5B10 were equally able to protect mice from a lethal challenge of E. coli 0111:B4 organisms, the outer core-specific IgG2a antibody was much more efficient at mediating the binding of human complement C3 than the O-antigen-specific IgG3 monoclonal antibody.

摘要

研究了用大肠杆菌0111:B4细菌免疫并用纯化脂多糖(LPS)加强免疫的小鼠产生的两种免疫保护性单克隆抗体的抗原特异性。其中一种抗体B7,经十二烷基硫酸钠聚丙烯酰胺凝胶电泳和免疫染色显示可与含O抗原的LPS种类结合,而另一种抗体5B10则与含O抗原的同源物和O抗原缺陷型LPS都有反应。5B10不与大肠杆菌J5(大肠杆菌0111:B4的Rc突变体,缺乏O抗原和外核心糖)的LPS结合。5B10不与其他几种大肠杆菌菌株的LPS发生交叉反应。因此,5B10似乎识别LPS外核心中一种不包括Rc决定簇的型特异性表位。对聚合O抗原特异的单克隆抗体属于IgG3亚类,对LPS外核心特异的单克隆抗体5B10是IgG2a。虽然B7和5B10同样能够保护小鼠免受大肠杆菌0111:B4菌株的致死性攻击,但外核心特异性IgG2a抗体在介导人补体C3结合方面比O抗原特异性IgG3单克隆抗体效率高得多。

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