Department of Pharmaceutical Microbiology, Faculty of Pharmacy and Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Microb Drug Resist. 2021 May;27(5):637-646. doi: 10.1089/mdr.2020.0130. Epub 2020 Sep 29.
The limitations of treatment options in bloodstream infections caused by multidrug-resistant (MDRAB) have been related to high morbidity and mortality. The aim of our present study was to determine antimicrobial susceptibility profiles, molecular resistance patterns, and biofilm properties of isolated from bloodstream infections. In the present study, a total of 44 bloodstream isolates were included. Antimicrobial susceptibility profiles and biofilm formation ability were assessed. The distribution of class D carbapenemases, IS, IS/OXA-23, NDM-1, , and was investigated by polymerase chain reaction (PCR). Arbitrarily primed-PCR (AP-PCR) was performed to evaluate clonal relationships. A total of 32 isolates were MDRAB, whereas 6 isolates were also resistant to colistin without positivity. All isolates were harboring OXA-51 gene, whereas OXA-23 positivity was 63.6%. Fifty percent of the isolates had IS IS upstream of OXA-23 was determined in 18 isolates. None of the isolates were positive for NDM-1 gene. Majority of the strains were strong biofilm producers (86.8%). A total of 56.8% of the isolates were positive for gene with no direct association with strong biofilm formation. However, OXA-51 + 23 genotype and trimethoprim-sulfamethoxazole resistance showed a significant relationship with biofilm formation. AP-PCR analysis revealed six distinct clusters of . Herein, majority of the blood isolates were characterized as OXA-51+OXA-23 carbapenemase genotype and were strong biofilm formers. None of the isolates were positive for NDM-1, which was promising. Resistant isolates were tended to form strong biofilms. Our results highlight the emergence of oxacillinase-producing MDRAB isolated from bloodstream with high biofilm formation ability.
血流感染中多药耐药 （MDRAB） 的治疗选择有限，这与高发病率和死亡率有关。我们目前的研究旨在确定从血流感染中分离出的 的抗菌药物敏感性谱、分子耐药模式和生物膜特性。在本研究中，共纳入了 44 株血流分离株。评估了抗菌药物敏感性谱和生物膜形成能力。通过聚合酶链反应（PCR）检测了 D 类碳青霉烯酶、IS、IS/OXA-23、NDM-1、 和 的分布。采用任意引物 PCR（AP-PCR）评估克隆关系。共 32 株为 MDRAB，6 株对多粘菌素耐药且 阴性。所有分离株均携带 OXA-51 基因，而 OXA-23 阳性率为 63.6%。18 株分离株中存在 OXA-23 上游的 IS。未检测到 NDM-1 基因阳性。大多数菌株为强生物膜生产者（86.8%）。共有 56.8%的分离株 基因阳性，与强生物膜形成无直接关联。然而，OXA-51+23 基因型和复方磺胺甲噁唑耐药与生物膜形成呈显著相关。AP-PCR 分析显示有 6 个不同的 聚类。本研究中，大多数血分离株表现为 OXA-51+OXA-23 碳青霉烯酶基因型，且为强生物膜形成者。未检测到 NDM-1 的阳性，这是有希望的。耐药分离株倾向于形成强生物膜。我们的研究结果强调了从血流中分离出的产耐甲氧西林酶的 MDRAB 具有高生物膜形成能力。
