[Determination of cell surface antigens on cryostat sections with monoclonal antibodies].

Most monoclonal antibodies recognize antigens which do not survive conventional tissue processing: the use of frozen tissue sections and the immunofluorescence method overcome this obstacle but introduce other problems. Three improvements are reported here: the use of serum-free (substitute) "Ultroser Hy" as a culture medium for hybridomas, in order to diminish background staining and the diffusion artifact; the use of freon for freezing tissue sections, so as to slightly increase cellular morphology and staining; the use of a new immunofluorescent slide-mounting medium to enhance histologic preservation and immunohistologic contrast and to diminish fading of immunofluorescence.