Department of Parasitology, Institute of Functional Biology and Ecology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Department of Parasitology, Institute of Functional Biology and Ecology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Dev Comp Immunol. 2021 Feb;115:103879. doi: 10.1016/j.dci.2020.103879. Epub 2020 Sep 29.
The mouse intestinal parasite Heligmosomoides polygyrus demonstrates adaptation to the inflammatory milieu as a result of colitis induced by dextran sulphate sodium (DSS). Nematodes from mice with colitis had different effects on dendritic cells than nematodes from mice without colitis. Immature JAWSII cells pre-exposed to L4 stage H. polygyrus from DSS-treated mice were adoptively transferred to mice with induced colitis. After two days, a higher disease activity index, macroscopic damage score and colon histology score were observed. MLN T cells isolated nine days after transfer demonstrated proinflammatory IFN-γ and IL-17 production. Transfer of JAWSII stimulated with male or female L4 larvae from a control invasion resulted in a slight improvement of colitis; in addition, dendritic cells exposed to H. polygyrus female L4 larvae, provoked migration of CD8CD25 T cells from MLN to the colon. Nematodes from an inflammatory environment changed cytokine production by dendritic cells. Inflammatory milieu changing nematode immunomodulatory activity affects dendritic cell functions, which offers new insight into the helminth-host relationship.
肠道寄生虫旋毛虫多形性表现出对炎症环境的适应，这是由于葡聚糖硫酸钠（DSS）诱导的结肠炎引起的。来自结肠炎小鼠的线虫与来自无结肠炎小鼠的线虫相比，对树突状细胞有不同的影响。预先用来自 DSS 处理的小鼠的 H. polygyrus L4 期幼虫暴露的未成熟 JAWSII 细胞被过继转移到诱导结肠炎的小鼠中。两天后，观察到更高的疾病活动指数、宏观损伤评分和结肠组织学评分。转移后九天从转移中分离出的 MLN T 细胞表现出促炎 IFN-γ 和 IL-17 产生。用来自对照入侵的雄性或雌性 L4 幼虫刺激的 JAWSII 转移导致结肠炎略有改善；此外，暴露于 H. polygyrus 雌性 L4 幼虫的树突状细胞促使来自 MLN 的 CD8CD25 T 细胞迁移到结肠。来自炎症环境的线虫改变了树突状细胞的细胞因子产生。改变线虫免疫调节活性的炎症环境会影响树突状细胞的功能，这为寄生虫与宿主的关系提供了新的见解。
