College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, PR China.
Department of Clinical Nutrition, Affiliated Hospital of Yangzhou University, Yangzhou 225012, PR China.
Int J Biol Macromol. 2020 Dec 15;165(Pt A):625-634. doi: 10.1016/j.ijbiomac.2020.09.209. Epub 2020 Sep 30.
Kaempferol, quercetin and myricetin are typical flavonols that feature different number of hydroxyl substituents at B-ring. In this study, kaempferol, quercetin and myricetin were individually mixed with chitosan (CS) based film matrix to develop active packaging films. Results showed the incorporation of flavonols produced dense inner microstructure by establishing intermolecular hydrogen bonding interactions with film matrix. The addition of flavonols reduced the water vapor permeability, oxygen permeability and UV-vis light transmittance of the films, whereas elevated the tensile strength, elongation at break, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activity of the films. Among all the films, the film containing myricetin showed the highest water vapor and oxygen barrier abilities and mechanical properties. Notably, different films presented distinct flavonol release behaviors in fatty and aqueous food stimulants. Kaempferol was easily released into fatty food stimulant, while myricetin was easily released into aqueous food stimulant. The release behavior of flavonols in different food stimulants greatly affected the antioxidant activity of the films. Our results suggest the structure and functional properties of the films are closely related to the number of hydroxyl substituents at B-ring of flavonols.
山奈酚、槲皮素和杨梅素是典型的黄酮醇，其 B 环上具有不同数量的羟基取代基。本研究将山奈酚、槲皮素和杨梅素分别与壳聚糖（CS）基膜基质混合，开发活性包装膜。结果表明，黄酮醇的掺入通过与膜基质建立分子间氢键相互作用，产生了致密的内部微观结构。黄酮醇的添加降低了膜的水蒸气透过率、氧气透过率和紫外可见光透过率，而提高了膜的拉伸强度、断裂伸长率、2,2-二苯基-1-苦基肼自由基清除活性和抗菌活性。在所有的膜中，含有杨梅素的膜表现出最高的水蒸气和氧气阻隔能力以及机械性能。值得注意的是，不同的膜在脂肪和水基食品刺激物中表现出不同的黄酮醇释放行为。山奈酚容易释放到脂肪性食品刺激物中，而杨梅素容易释放到水性食品刺激物中。黄酮醇在不同食品刺激物中的释放行为极大地影响了膜的抗氧化活性。我们的结果表明，膜的结构和功能性质与黄酮醇 B 环上的羟基取代基数量密切相关。
