Chen Qian-Li, Cai Lin, Wang Han-Cheng, Cai Liu-Ti, Goodwin Paul, Ma Jun, Wang Feng, Li Zhong
Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang, China.
College of Agriculture, Guizhou University, Guiyang, China.
Front Microbiol. 2020 Sep 4;11:554051. doi: 10.3389/fmicb.2020.554051. eCollection 2020.
causes tobacco pole rot in China during tobacco flue-curing. Flue-curing is a post-harvest process done to prepare tobacco leaves and involves three different stages: the yellowing stage has the lowest temperatures and highest humidity, then the color-fixing stage has higher temperatures and medium humidity, and finally the stem-drying stage has the highest temperatures and lowest humidity. In this study, fungal culturing and IonS5XL high-throughput sequencing techniques were used to reveal the fungal community of the petioles and lamina of tobacco leaves infected with pole rot during flue-curing. A total of 108 fungal isolates belonging to 6 genera were isolated on media. The most common fungal species isolated was the pathogen, , that was most often found equally on petioles and laminas in the color-fixing stage, followed by saprotrophs, mostly spp. High-throughput sequencing revealed saprotrophs with being the most abundant genus, followed by , , and , whereas was the tenth most abundant genus, which was mostly found on petioles at the yellowing stage. Both culturable fungal diversity and fungal sequence diversity was higher at stem-drying stage than the yellowing and color-fixing stages, and diversity was higher with leaf lamina than petioles revealing that the changes in fungal composition and diversity during the curing process were similar with both methods. This study demonstrates that the curing process affects the leaf microbiome of tobacco during the curing process, and future work could examine if any of these saprotrophic fungi detected during the curing of tobacco leaves may be potential biocontrol agents for with pole rot in curing chambers.
在中国，它会导致烤烟过程中烟杆腐烂。烤烟是一种用于制备烟叶的采后加工过程，包括三个不同阶段：变黄阶段温度最低、湿度最高，接着定色阶段温度较高、湿度适中，最后干筋阶段温度最高、湿度最低。在本研究中，采用真菌培养和IonS5XL高通量测序技术来揭示烤烟过程中感染烟杆腐烂的烟叶叶柄和叶片的真菌群落。在培养基上共分离出属于6个属的108株真菌分离物。分离出的最常见真菌种类是病原菌，在定色阶段，它在叶柄和叶片上出现的频率通常相同，其次是腐生菌，主要是 spp.。高通量测序显示腐生菌中 属最为丰富，其次是 、 、 和 ，而 属是第十丰富的属，主要在变黄阶段的叶柄上发现。可培养真菌多样性和真菌序列多样性在干筋阶段均高于变黄和定色阶段，且叶片的多样性高于叶柄，这表明两种方法揭示的烘烤过程中真菌组成和多样性的变化相似。本研究表明，烘烤过程会影响烘烤过程中烟叶的叶微生物组，未来的工作可以研究在烟叶烘烤过程中检测到的这些腐生真菌中是否有任何一种可能是烘烤室中防治烟杆腐烂的潜在生物防治剂。
