SLPI 通过调节片状伪足/皱襞和桥粒来促进细胞迁移，Galectin4 在其中发挥重要作用。
SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
机构信息
Division of Microscopic Anatomy, Niigata University Graduate School of Medical and Dental Sciences , Niigata-shi, Japan.
Office of Institutional Research, Hokkaido University , Kita-ku, Japan.
出版信息
Cell Adh Migr. 2020 Dec;14(1):195-203. doi: 10.1080/19336918.2020.1829264.
To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal ruffles were smaller and moved slower in ΔSLPI cells compared to wtCa9-22 cells. Furthermore, well-developed intermediate filament bundles were observed at the desmosome junction of ΔSLPI cells. In addition, was strongly expressed in ΔSLPI cells, and its forced expression suppressed migration of wtCa9-22 cells. Taken together, SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
为了阐明分泌白细胞蛋白酶抑制剂 (SLPI) 诱导细胞迁移的潜在机制，我们使用几种显微镜成像方法和基因表达分析比较了 SLPI 缺失的人牙龈癌细胞 Ca9-22（ΔSLPI）和原始（野生型：wt）Ca9-22 细胞。结果表明，与 wtCa9-22 细胞相比，ΔSLPI 细胞的迁移减少。与 wtCa9-22 细胞相比，ΔSLPI 细胞的片状伪足/背侧皱襞更小，移动更慢。此外，在 ΔSLPI 细胞的桥粒连接处观察到发育良好的中间丝束。此外，在 ΔSLPI 细胞中强烈表达，其强制表达抑制 wtCa9-22 细胞的迁移。总之，SLPI 通过调节片状伪足/皱襞和桥粒来促进细胞迁移，其中半乳糖凝集素 4 起着重要作用。
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