Bioengineering Graduate Program, Pennsylvania State University, University Park, United States.
Molecular, Cellular, and Integrative Biology Graduate Program, Pennsylvania State University, University Park, United States.
Elife. 2020 Oct 5;9:e60533. doi: 10.7554/eLife.60533.
Cortical neural activity is coupled to local arterial diameter and blood flow. However, which neurons control the dynamics of cerebral arteries is not well understood. We dissected the cellular mechanisms controlling the basal diameter and evoked dilation in cortical arteries in awake, head-fixed mice. Locomotion drove robust arterial dilation, increases in gamma band power in the local field potential (LFP), and increases calcium signals in pyramidal and neuronal nitric oxide synthase (nNOS)-expressing neurons. Chemogenetic or pharmocological modulation of overall neural activity up or down caused corresponding increases or decreases in basal arterial diameter. Modulation of pyramidal neuron activity alone had little effect on basal or evoked arterial dilation, despite pronounced changes in the LFP. Modulation of the activity of nNOS-expressing neurons drove changes in the basal and evoked arterial diameter without corresponding changes in population neural activity.
皮质神经元活动与局部动脉直径和血流相关联。然而，控制脑动脉动力学的神经元尚不清楚。我们在清醒、头部固定的小鼠中分离控制皮质动脉基础直径和诱发扩张的细胞机制。运动驱动动脉显著扩张，局部场电位 (LFP) 中的伽马波段功率增加，以及锥体神经元和神经元型一氧化氮合酶 (nNOS) 表达神经元中的钙信号增加。整体神经元活动的化学遗传或药理学调节增加或减少导致基础动脉直径相应增加或减少。尽管 LFP 发生明显变化，但单独调节锥体神经元活动对基础或诱发动脉扩张几乎没有影响。nNOS 表达神经元活性的调节驱动基础和诱发动脉直径的变化，而没有相应的群体神经元活动变化。
