静脉血和毛细血管血中β淀粉样肽的分析前采样和存储条件。

Pre-Analytical Sampling and Storage Conditions of Amyloid-β Peptides in Venous and Capillary Blood.

机构信息

University Medical Center Goettingen (UMG), Georg-August-University, Department of Psychiatry and Psychotherapy, Goettingen, Germany.

German Center for Neurodegenerative Diseases (DZNE), Goettingen, Germany.

出版信息

J Alzheimers Dis. 2020;78(2):529-535. doi: 10.3233/JAD-200777.

DOI:10.3233/JAD-200777

PMID:33016918

Abstract

Previous studies on blood-based biomarkers for Alzheimer's disease suggest a less invasive blood test might be a valuable screening tool for Alzheimer-specific pathology. Pre-analytical sample storage conditions seem to play an important role on amyloid-β (Aβ) stability, impacting reliability and reproducibility. This study shows that Aβ40, Aβ42, and Aβ42/40 levels significantly and early decrease during storage at room temperature in whole blood or plasma. Storing blood samples at 4°C leads to stable Aβ peptide concentrations up to 72 h. In addition, Aβ peptides can be measured in capillary blood with a stable Aβ42/40 ratio up to 72 h at 4°C.

摘要

先前关于阿尔茨海默病血液生物标志物的研究表明，侵入性更小的血液检测可能成为阿尔茨海默病特定病理的有效筛查工具。在分析前的样本储存条件似乎对淀粉样蛋白-β（Aβ）的稳定性起着重要作用，从而影响检测的可靠性和可重复性。本研究表明，在室温下全血或血浆中储存时，Aβ40、Aβ42 和 Aβ42/40 水平会显著且早期下降。将血液样本储存在 4°C 可使 Aβ 肽浓度稳定长达 72 小时。此外，Aβ 肽可在 4°C 下于毛细血管血中测量，Aβ42/40 比值稳定长达 72 小时。