Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, Thailand.
Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
PLoS One. 2020 Oct 5;15(10):e0238939. doi: 10.1371/journal.pone.0238939. eCollection 2020.
Long-term use of colistin for preventing Gram-negative bacterial infections in food animals was prohibited in Thailand in 2017, but it is permitted for short-term treatment. This study aimed to investigate association between the use of colistin for short-term treatment of infection and the emergence of colistin-resistant Enterobacteriaceae in swine. The current study was conducted at 2 selected swine farms in Thailand. Neither farm has used colistin to prevent infection for longer than 1 year. Rectal swabs were collected from the same 66 pigs at birth, and on days 7, 14, 21, 28, and 60. Colistin was used to treat sick pigs for up to 3 days. Additional rectal swabs were collected during colistin treatment. Rectal swabs were analyzed for colistin-resistant Enterobacteriaceae and the mcr-1 gene. Results revealed that colistin-resistant Enterobacteriaceae were absent at birth. Some pigs at both farms had diarrhea and received colistin treatment during days 2-27. Colistin-resistant Enterobacteriaceae were detected in 13.3-50.0% of sick and healthy pigs. No sick pigs were observed during days 28-60, and colistin was not used during that period. Colistin-resistant Enterobacteriaceae were detected in 2.8-10.0% of healthy pigs on day 28, and in 0-3.4% of healthy pigs on day 60. The mcr-1 gene was detected in 57.6% of colistin-resistant Enterobacteriaceae isolates. Short-term treatment with colistin was found to be associated with the emergence of colistin-resistant Enterobacteriaceae in swine. Colistin-resistant Enterobacteriaceae rapidly emerged after colistin use, and rapidly decreased or disappeared after its discontinuation.
2017 年，泰国禁止在食用动物中长期使用黏菌素预防革兰氏阴性细菌感染，但允许短期治疗。本研究旨在调查短期治疗感染时使用黏菌素与猪中产黏菌素耐药肠杆菌科细菌的出现之间的关系。本研究在泰国的 2 个选定的猪场进行。这两个猪场都没有使用黏菌素来预防感染超过 1 年。在出生时、第 7、14、21、28 和 60 天，从相同的 66 头猪中采集直肠拭子。当猪生病时，使用黏菌素治疗最多 3 天。在使用黏菌素治疗期间采集额外的直肠拭子。对直肠拭子进行分析以确定是否存在产黏菌素耐药肠杆菌科细菌和 mcr-1 基因。结果表明，出生时不存在产黏菌素耐药肠杆菌科细菌。两个猪场的一些猪在第 2-27 天出现腹泻并接受黏菌素治疗。在生病和健康的猪中，有 13.3-50.0%检测到产黏菌素耐药肠杆菌科细菌。在第 28-60 天没有观察到生病的猪，并且在此期间没有使用黏菌素。在第 28 天，2.8-10.0%的健康猪和第 60 天 0-3.4%的健康猪检测到产黏菌素耐药肠杆菌科细菌。mcr-1 基因在 57.6%的产黏菌素耐药肠杆菌科细菌分离株中被检测到。短期治疗使用黏菌素与猪中产黏菌素耐药肠杆菌科细菌的出现有关。在使用黏菌素后，产黏菌素耐药肠杆菌科细菌迅速出现，在停止使用后迅速减少或消失。
