脑脊液中无细胞DNA水平升高预示血液系统恶性肿瘤软脑膜播散
High Cell-Free DNA Levels in Cerebrospinal Fluid Predict Leptomeningeal Seeding of Hematologic Malignancy.
作者信息
Kim Eun Young, Lee Soon Tae, Kim Young Sook, Byun Ja Min, Hong Junshik, Shin Dong Yeop, Koh Youngil, Kim Inho
机构信息
Department of Neurology, Seoul National University Hospital, Seoul, Korea.
Division of Hematology/Oncology, Department of Internal Medicine, Seoul National University Hospital, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
出版信息
J Clin Neurol. 2020 Oct;16(4):581-585. doi: 10.3988/jcn.2020.16.4.581.
BACKGROUND AND PURPOSE
The main difficulty when diagnosing leptomeningeal metastases (LMSs) is the low sensitivity of cytology. Cancer cells release cell-free DNA (cfDNA) during proliferation and apoptosis, and so we analyzed the cfDNA level as a biomarker for LMSs in hematologic malignancy.
METHODS
This study prospectively enrolled 20 patients with hematologic malignancy who underwent cerebrospinal fluid (CSF) analysis. LMS was diagnosed based on both CSF cytology and clinical findings.
RESULTS
The CSF level of cfDNA was higher in patients with LMSs (108.17±84.84 ng/mL, mean±standard deviation) than in non-LMS patients (14.23±2.78 ng/mL). The sensitivity of cfDNA was higher than that of cytology (100% vs. 87%).
CONCLUSIONS
The cfDNA level in the CSF can be used as a supplemental marker for diagnosing LMS in hematologic malignancy patients.
背景与目的
诊断软脑膜转移（LMS）时的主要困难在于细胞学检查的敏感性较低。癌细胞在增殖和凋亡过程中会释放游离DNA（cfDNA），因此我们分析了cfDNA水平作为血液系统恶性肿瘤中LMS的生物标志物。
方法
本研究前瞻性纳入了20例接受脑脊液（CSF）分析的血液系统恶性肿瘤患者。LMS根据CSF细胞学检查和临床发现进行诊断。
结果
LMS患者的CSF中cfDNA水平（108.17±84.84 ng/mL，平均值±标准差）高于非LMS患者（14.23±2.78 ng/mL）。cfDNA的敏感性高于细胞学检查（100%对87%）。
结论
CSF中的cfDNA水平可作为诊断血液系统恶性肿瘤患者LMS的补充标志物。
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