Watanabe T, Nakai Y
Arch Histol Jpn. 1987 Mar;50(1):103-12. doi: 10.1679/aohc.50.103.
The synaptic interactions between catecholaminergic terminals and luteinizing hormone releasing hormone (LHRH)-containing neurons in the medial preoptic area of the rat was studied by electron microscopy using LHRH immunocytochemistry combined with 5-hydroxydopamine labeling or autoradiography after injection of 3H-dopamine or 3H-noradrenaline in the same tissue section. Axon terminals labeled with 5-hydroxydopamine, 3H-dopamine or 3H-noradrenaline were found to make synapse-like contacts with LHRH-immunoreactive nerve cell bodies and fibers in the medial preoptic area and also 5-hydroxydopamine-labeled terminals made synaptic contacts with the same LHRH-immunoreactive nerve fibers with unlabeled terminals. These findings suggest that catecholaminergic neurons may innervate LHRH-containing neurons to regulate LHRH secretion via synapses with other unknown neurons in the medial preoptic area of the rat.
运用电子显微镜，结合LHRH免疫细胞化学技术，对注射了3H-多巴胺或3H-去甲肾上腺素的同一组织切片进行5-羟多巴胺标记或放射自显影，研究了大鼠视前内侧区儿茶酚胺能终末与含促黄体生成素释放激素（LHRH）神经元之间的突触相互作用。发现用5-羟多巴胺、3H-多巴胺或3H-去甲肾上腺素标记的轴突终末，与视前内侧区LHRH免疫反应性神经细胞体和纤维形成类似突触的接触，并且5-羟多巴胺标记的终末与相同的LHRH免疫反应性神经纤维以及未标记的终末形成突触接触。这些发现表明，儿茶酚胺能神经元可能通过与大鼠视前内侧区其他未知神经元形成突触来支配含LHRH的神经元，从而调节LHRH的分泌。
