一种用于灵敏检测微小RNA的超支化转录激活CRISPR-Cas12a信号放大策略。
A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing.
作者信息
Tian Weimin, Liu Xiaoling, Wang Gaoting, Liu Chenghui
机构信息
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, Shaanxi Province, P. R. China.
出版信息
Chem Commun (Camb). 2020 Nov 11;56(87):13445-13448. doi: 10.1039/d0cc06034h. Epub 2020 Oct 12.
A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies.
开发了一种基于CRISPR-Cas12a的策略用于灵敏的微小RNA检测，其中将超支化滚环扩增和转录整合以激活Cas12a的反式切割能力。整合的三重信号放大赋予该策略低背景和高灵敏度。这种设计扩展了基于CRISPR-Cas的传感工具箱的范围，并在生物学和生物医学研究中显示出巨大潜力。
Zotero 插件