通过高效液相色谱法分离血红蛋白和血红蛋白链。

Separation of hemoglobins and hemoglobin chains by high-performance liquid chromatography.

作者信息

Huisman T H

出版信息

J Chromatogr. 1987 Jul 17;418:277-304. doi: 10.1016/0378-4347(87)80012-9.

Abstract

This paper evaluates various high-performance liquid chromatographic (HPLC) procedures useful for the detection and evaluation of numerous hemoglobin abnormalities in adults and in newborn infants. Two major approaches are discussed. The first concerns the analyses of hemoglobins in red cell lysates by anion- and cation-exchange chromatography; this type of HPLC has great potential in a routine laboratory, as quantitative data for numerous abnormalities can readily be obtained. The second concerns the separation of globin chains by reversed-phase HPLC that is most useful for the identification of hemoglobinopathies in adults, newborns, and fetuses.

摘要

本文评估了多种高效液相色谱（HPLC）方法，这些方法可用于检测和评估成人及新生儿中多种血红蛋白异常情况。文中讨论了两种主要方法。第一种涉及通过阴离子交换色谱法和阳离子交换色谱法分析红细胞裂解液中的血红蛋白；这种类型的HPLC在常规实验室中具有很大潜力，因为可以很容易地获得多种异常情况的定量数据。第二种涉及通过反相HPLC分离珠蛋白链，这对于鉴定成人、新生儿和胎儿中的血红蛋白病最为有用。