Luo Shan-Wei, Alimujiang Adili, Balamurugan Srinivasan, Zheng Jian-Wei, Wang Xiang, Yang Wei-Dong, Cui Jianghu, Li Hong-Ye
Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.
J Hazard Mater. 2021 Feb 15;404(Pt B):124014. doi: 10.1016/j.jhazmat.2020.124014. Epub 2020 Oct 1.
Molybdenum disulfide nanoparticles (MoS NPs) has emerged as the promising nanomaterial with a wide array of applications in the biomedical, industrial and environmental field. However, the potential effect of MoS NPs on marine organisms has yet to be reported. In this study, the effect of MoS NPs on the physiological index, subcellular morphology, transcriptomic profiles of the marine microalgae Dunaliella salina was investigated for the first time. exhibited "doping-like" effects on marine microalgae; Growth stimulation was 193.55%, and chlorophyll content increased 1.61-fold upon the addition of 50 μg/L MoS NPs. Additionally, exposure to MoS NPs significantly increased the protein and carbohydrate content by 2.03- and 1.56-fold, respectively. The antioxidant system was activated as well to eliminate the adverse influence of reactive oxygen species (ROS). Transcriptomic analysis revealed that genes involved in porphyrin synthesis, glycolysis/gluconeogenesis, tricarboxylic acid cycle and DNA replication were upregulated upon MoS NPs exposure, which supports the mechanistic role of MoS NPs in improving cellular growth and photosynthesis. The "doping-like" effects on marine algae suggest that the low concentration of MoS NPs might change the rudimentary ecological composition in the ocean.
二硫化钼纳米颗粒（MoS NPs）已成为一种有前景的纳米材料，在生物医学、工业和环境领域有广泛应用。然而，MoS NPs对海洋生物的潜在影响尚未见报道。在本研究中，首次研究了MoS NPs对海洋微藻杜氏盐藻生理指标、亚细胞形态和转录组图谱的影响。对海洋微藻表现出“类掺杂”效应；添加50μg/L的MoS NPs后，生长刺激率为193.55%，叶绿素含量增加了1.61倍。此外，暴露于MoS NPs显著提高了蛋白质和碳水化合物含量，分别增加了2.03倍和1.56倍。抗氧化系统也被激活以消除活性氧（ROS）的不利影响。转录组分析表明，暴露于MoS NPs后，参与卟啉合成、糖酵解/糖异生、三羧酸循环和DNA复制的基因上调，这支持了MoS NPs在改善细胞生长和光合作用中的作用机制。对海洋藻类的“类掺杂”效应表明，低浓度的MoS NPs可能会改变海洋中的基本生态组成。
