海星 Asterina pectinifera：成体的采集和养护以及幼虫的培育和变态。

The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae.

机构信息

Department of Physiology, Teikyo University School of Medicine, Itabashi-ku, Tokyo, Japan.

School of Life Science and Technology, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.

出版信息

Methods Mol Biol. 2021;2219:49-68. doi: 10.1007/978-1-0716-0974-3_3.

DOI:10.1007/978-1-0716-0974-3_3

PMID:33074533

Abstract

Here we describe methods for (a) collecting starfish during their breeding period; (b) maintaining adults with fully grown gonads in laboratory aquaria; (c) rearing fertilized eggs to brachiolaria larvae, and (d) inducing larvae to metamorphose into juveniles under laboratory conditions. Such protocols should facilitate various analyses of starfish development throughout the entire life cycle of these model organisms.

摘要

在此，我们介绍了在繁殖期采集海星的方法：（a）；（b）在实验室水族箱中维持性腺完全成熟的成体；（c）将受精卵培育成腕幼虫；（d）在实验室条件下诱导幼虫变态为幼体。这些方案将有助于对这些模式生物整个生命周期中的海星发育进行各种分析。