基因多态性与中国人群类风湿关节炎的相关性。
Association of Gene Polymorphisms with Rheumatoid Arthritis in a Chinese Population.
机构信息
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, China.
Anhui Provincial Laboratory of Inflammatory and Immune Diseases, 81 Meishan Road, Hefei, Anhui, China.
出版信息
Biomed Res Int. 2020 Oct 6;2020:3789319. doi: 10.1155/2020/3789319. eCollection 2020.
BACKGROUND
Recently, increasing studies have revealed that leptin is involved in the development of rheumatoid arthritis (RA). This study is aimed at exploring the association of gene single nucleotide polymorphisms (SNPs) with susceptibility to RA in a Chinese population.
METHODS
We recruited 600 RA patients and 600 healthy controls from a Chinese population and analyzed their three SNPs (rs10244329, rs2071045, and rs2167270) using the improved Multiplex Ligase Detection Reaction (iMLDR) assays. The associations of these SNPs with clinical manifestations of RA were also analyzed. Enzyme-linked immunosorbent assay (ELISA) was performed for plasma determination.
RESULTS
No significant difference in either allele or genotype frequencies of these three SNPs between RA patients and healthy controls was observed (all > 0.05). Association between the genotype effects of dominant, recessive models was also not found (all > 0.05). No significant difference in plasma levels was detected between RA patients and controls ( > 0.05).
CONCLUSION
gene (rs10244329, rs2071045, and rs2167270) polymorphisms are not associated with RA genetic susceptibility and its clinical features in the Chinese population.
背景
最近，越来越多的研究表明瘦素参与了类风湿关节炎（RA）的发生发展。本研究旨在探讨中国人群中基因单核苷酸多态性（SNPs）与 RA 易感性的相关性。
方法
我们从中国人群中招募了 600 名 RA 患者和 600 名健康对照者，采用改良多重连接酶检测反应（iMLDR）检测了他们的三个 SNP（rs10244329、rs2071045 和 rs2167270）。还分析了这些 SNP 与 RA 临床表现的相关性。采用酶联免疫吸附测定（ELISA）法测定血浆。
结果
RA 患者和健康对照组之间这三个 SNP 的等位基因或基因型频率均无显著差异（均>0.05）。显性、隐性模型的基因型效应之间也没有关联（均>0.05）。RA 患者和对照组之间的血浆水平无显著差异（>0.05）。
结论
在中国人群中，基因（rs10244329、rs2071045 和 rs2167270）多态性与 RA 的遗传易感性及其临床特征无关。
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