Huang Bixing, Yang Qiong, Hoffmann Ary A, Ritchie Scott A, van den Hurk Andrew F, Warrilow David
Public Health Virology Laboratory, Queensland Health Forensic and Scientific Services, PO Box 594, Archerfield 4108, Australia.
Pest and Environmental Adaptation Research Group (PEARG), School of BioSciences, Bio21 Institute, The University of Melbourne, Parkville 3052, Australia.
iScience. 2020 Sep 16;23(10):101572. doi: 10.1016/j.isci.2020.101572. eCollection 2020 Oct 23.
A dengue suppression strategy based on release of mosquitoes infected with the bacterium is being trialed in many countries. inhibits replication and transmission of dengue viruses. Questions remain regarding the long-term stability of virus-suppressive effects. We sequenced the genome and analyzed mitochondrial DNA markers isolated from mosquitoes sampled 2-8 years after releases in the greater Cairns region, Australia. Few changes were detected when genomes of field mosquitoes were compared with genomes of mosquitoes obtained soon after initial releases. Mitochondrial variants associated with the initial release stock are now the only variants found in release sites, highlighting maternal leakage as a possible explanation for rare -negative mosquitoes and not migration from non-release areas. There is no evidence of changes in the genome that indicate selection against its viral-suppressive effects or other phenotypes attributable to infection with the bacterium.
一种基于释放感染该细菌的蚊子的登革热抑制策略正在许多国家进行试验。该细菌可抑制登革热病毒的复制和传播。关于病毒抑制效果的长期稳定性仍存在疑问。我们对澳大利亚凯恩斯地区释放蚊子2至8年后采集的蚊子的基因组进行了测序，并分析了分离出的线粒体DNA标记。将野外蚊子的基因组与初次释放后不久获得的蚊子的基因组进行比较时，未检测到明显变化。与初次释放种群相关的线粒体变异现在是释放地点发现的唯一变异，这突出表明母系渗漏是罕见的细菌阴性蚊子出现的可能原因，而非来自非释放区域的迁移。没有证据表明基因组发生了变化，表明对其病毒抑制作用或因感染该细菌而产生的其他表型存在选择。
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