National Research Center for Veterinary Medicine, Luoyang, P.R. China.
Monoclon Antib Immunodiagn Immunother. 2020 Oct;39(5):184-189. doi: 10.1089/mab.2020.0032.
Foot-and-mouth disease (FMD) is caused by FMD virus (FMDV) is a highly contagious disease of ruminants, which is primarily controlled by vaccination. The monitoring of antisera after vaccination is currently depending on liquid-phase blocking ELISA (LPBE). Recently, bacterium-original FMD virus-like particle (VLP) showed the potential as vaccine candidates. In this study, to minimize the risk of live virus involvement, the original VLP of FMDV serotype O were used as the immunogen for monoclonal antibodies (Mabs) production and the capture antigen in the development of a solid-phase competition ELISA (SPCE). The samples with a percentage inhibition of >50% were considered positive in the SPCE assay. The concordance rate of the Mab-based SPCE compared with the LPBE for clinical serum samples test was 93.4%, and with a high agreement (kappa = 0.892) with LPBE in antibody duration monitoring. Results indicated that the VLP-based SPCE had high specificity and sensitivity, which provides an alternative method for postimmunization antibody evaluation of FMDV serotype O.
口蹄疫（FMD）由口蹄疫病毒（FMDV）引起，是一种高度接触传染性反刍动物疾病，主要通过疫苗接种来控制。目前，疫苗接种后的血清监测依赖于液相阻断 ELISA（LPBE）。最近，细菌来源的口蹄疫病毒样颗粒（VLP）显示出作为疫苗候选物的潜力。在这项研究中，为了最大程度降低活病毒感染的风险，使用口蹄疫病毒血清型 O 的原始 VLP 作为单克隆抗体（Mab）产生的免疫原和固相竞争 ELISA（SPCE）中的捕获抗原。在 SPCE 检测中，抑制率大于 50%的样本被认为是阳性。Mab 为基础的 SPCE 与 LPBE 对临床血清样本检测的一致性率为 93.4%，与 LPBE 的抗体持续时间监测具有高度一致性（kappa=0.892）。结果表明，基于 VLP 的 SPCE 具有高特异性和敏感性，为 FMDV 血清型 O 的免疫后抗体评估提供了一种替代方法。
