维多利亚和火焰无核葡萄品种在浆果成熟过程中的表型和转录组比较分析。

Comparative phenotypic and transcriptomic analysis of Victoria and flame seedless grape cultivars during berry ripening.

机构信息

College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi, China.

Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China.

出版信息

FEBS Open Bio. 2020 Dec;10(12):2616-2630. doi: 10.1002/2211-5463.12996. Epub 2020 Nov 4.

DOI:10.1002/2211-5463.12996

PMID:33090714

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7714085/

Abstract

Grape berry development is a highly coordinated and intricate process. Herein, we analyzed the phenotypic and transcriptomic patterns of Victoria (VT) and Flame Seedless (FS) grape varieties during berry development. Physiological analysis and transcriptomic sequencing were performed at four berry developmental phases. VT berry size was comparatively larger to the FS variety. At maturity, 80 days postanthesis (DPA), the FS soluble solids were 61.8% higher than VT. Further, 4889 and 2802 differentially expressed genes were identified from VT and FS 40 DPA to 80 DPA development stages, respectively. VvSWEET15, VvHXK, and MYB44 genes were up-regulated during the postanthesis period, while bHLH14, linked to glucose metabolism, was gradually down-regulated during berry development. These genes may have significant roles in berry development, ripening, and sugar accumulation.

摘要

葡萄浆果发育是一个高度协调和复杂的过程。在此，我们分析了维多利亚（VT）和火焰无核（FS）葡萄品种在浆果发育过程中的表型和转录组模式。在四个浆果发育阶段进行了生理分析和转录组测序。VT 葡萄浆果的大小比 FS 品种大。在成熟时，授粉后 80 天（DPA），FS 的可溶性固形物比 VT 高 61.8%。此外，分别从 VT 和 FS 的 40 DPA 到 80 DPA 发育阶段鉴定了 4889 和 2802 个差异表达基因。在授粉后时期，VvSWEET15、VvHXK 和 MYB44 基因上调表达，而与葡萄糖代谢相关的 bHLH14 基因在浆果发育过程中逐渐下调。这些基因可能在浆果发育、成熟和糖积累过程中具有重要作用。