Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
Lead Contact.
STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100008. Epub 2020 Jun 3.
Glioblastoma (GBM) remains a devastating disease with a median survival of less than two years. Current preclinical models are unable to accurately reflect the complexity of human GBM. We recently established a cerebral organoid glioma (GLICO) model to study the invasion and biology of patient-derived glioma stem cells in miniature replicas of the human brain. Through the dissemination of our detailed methodology, we aim to encourage other scientists to further build upon our existing model for studying these destructive tumors. For complete details on the use and execution of this protocol, please refer to Linkous et al. (2019).
胶质母细胞瘤（GBM）仍然是一种致命的疾病，中位生存期不到两年。目前的临床前模型无法准确反映人类 GBM 的复杂性。我们最近建立了一个脑类器官胶质母细胞瘤（GLICO）模型，以研究患者来源的胶质母细胞瘤干细胞在人类大脑微型复制品中的侵袭和生物学特性。通过传播我们详细的方法，我们希望鼓励其他科学家进一步在我们现有的模型基础上研究这些破坏性肿瘤。有关使用和执行此方案的完整详细信息，请参阅 Linkous 等人（2019 年）。
