循环 T 细胞在肥胖症及肥胖相关糖尿病患者中表现出不同的 TIM3/Galectin-9 表达。
Circulating T Cells Exhibit Different TIM3/Galectin-9 Expression in Patients with Obesity and Obesity-Related Diabetes.
机构信息
Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical Immunology, First Affiliated Hospital of Soochow University, No. 708 Renmin Road, Suzhou, 215006 Jiangsu, China.
Departments of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou, 215006 Jiangsu, China.
出版信息
J Diabetes Res. 2020 Oct 15;2020:2583257. doi: 10.1155/2020/2583257. eCollection 2020.
AIMS
Obesity is highly associated with type 2 diabetes mellitus (T2DM). The TIM3/galectin-9 pathway plays an important role in immune tolerance. Herein, we aimed to investigate the expression of TIM3 and galectin-9 in peripheral blood and to evaluate their clinical significance in patients with obesity and obesity-related T2DM.
METHODS
We performed flow cytometry on peripheral blood samples from healthy donors (HC), patients with simple obesity (OB), and patients with obesity comorbid T2DM (OD). The expression of TIM3 on CD3, CD4, and CD8 T cells was determined. The level of galectin-9 in plasma was detected by ELISA.
RESULTS
We demonstrated the enhancement of TIM3 on CD3, CD4, and CD8 T cells in the OB group when compared with healthy controls, while it was decreased significantly in the OD group. The TIM3CD8 T cells of the OB group were positively correlated with risk factors including BMI, body fat rate, and hipline. The concentration of galectin-9 of the OD group in plasma was significantly higher than that of healthy donors and the OB group. Moreover, the level of galectin-9 of the OD group was positively correlated with fasting insulin and C-peptide, which were two clinical features that represented pancreatic islet function in T2DM.
CONCLUSIONS
Our results suggested that TIM3 and galectin-9 may be potential biomarkers related to the pathogenesis of obesity-related T2DM.
目的
肥胖与 2 型糖尿病（T2DM）高度相关。TIM3/半乳糖凝集素-9 途径在免疫耐受中发挥重要作用。本研究旨在检测肥胖症及肥胖相关 T2DM 患者外周血中 TIM3 和半乳糖凝集素-9 的表达，并评估其临床意义。
方法
我们采用流式细胞术检测了健康对照者（HC）、单纯性肥胖症（OB）和肥胖合并 T2DM 患者（OD）外周血样本中 TIM3 的表达。采用 ELISA 法检测血浆中半乳糖凝集素-9 的水平。
结果
与健康对照组相比，OB 组 CD3、CD4 和 CD8 T 细胞上 TIM3 的表达增强，而 OD 组 TIM3 的表达显著降低。OB 组的 TIM3+CD8 T 细胞与 BMI、体脂率和臀围等危险因素呈正相关。OD 组血浆中半乳糖凝集素-9 的浓度明显高于健康对照组和 OB 组。此外，OD 组半乳糖凝集素-9 的水平与空腹胰岛素和 C 肽呈正相关，而后者是 T2DM 中代表胰岛功能的两个临床特征。
结论
我们的研究结果表明，TIM3 和半乳糖凝集素-9 可能是与肥胖相关 T2DM 发病机制相关的潜在生物标志物。
Zotero 插件