Fang Wen-Ye, Li Xiang, Huang Yong, Guo Chao-Ran, Hu Yu-Ting, Tao Ren-Jie
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China.
Huan Jing Ke Xue. 2020 Aug 8;41(8):3699-3706. doi: 10.13227/j.hjkx.202002055.
In order to enhance the removal of NO-N in the ANAMMOX process, an element sulfur-based autotrophic short-cut denitrification (short-cut S-SADN) was introduced by adding elemental sulfur to an ANAMMOX continuous flow reactor. The effects of different influent NH-N/NO-N ratios on the nitrogen conversion and NO-N competitive characteristics in the coupled system were investigated at (33±2)℃ and a pH of 7.8-8.2. The results showed that under different influent NH-N/NO-N ratios (1:1.3, 1:1.5, 1:1, and 1:1.1), the average total nitrogen (TN) removal efficiency of the coupled system reached 96.78%, 97.21%, 94.68%, and 97.72%, respectively, which were much higher than the highest TN removal efficiency of the ANAMMOX theory (89%). Among them, the stable operation of deep nitrogen removal of the short-cut S-SADN coupled with ANAMMOX was successfully achieved with an influent NH-N/NO-N ratio of 1:1 or 1:1.1. Under the optimal influent NH-N/NO-N ratio of 1:1.1, the concentrations of influent NH-N and NO-N were 240 mg·L and 265 mg·L, respectively, the TN removal rate reached 1.50 kg·(m·d), and the TN removal efficiency of ANAMMOX and S-SADN pathways were stable at (95.68±1.22)% and (2.04±0.77)%, respectively. During the entire operational process, ANAMMOX always occupied an absolute advantage in the competition of substrate NO-N, and the activity of ANAMMOX bacteria (NH-N/VSS) was stable at (0.166±0.008)kg·(kg·d).
为提高厌氧氨氧化工艺中NO-N的去除效果，通过向厌氧氨氧化连续流反应器中添加单质硫，引入了基于单质硫的自养型短程反硝化（短程S-SADN）。在(33±2)℃、pH为7.8 - 8.2的条件下，研究了不同进水NH-N/NO-N比对耦合系统中氮转化及NO-N竞争特性的影响。结果表明，在不同进水NH-N/NO-N比（1:1.3、1:1.5、1:1和1:1.1）下，耦合系统的平均总氮（TN）去除效率分别达到96.78%、97.21%、94.68%和97.72%，远高于厌氧氨氧化理论的最高TN去除效率（89%）。其中，进水NH-N/NO-N比为1:1或1:1.1时，成功实现了短程S-SADN与厌氧氨氧化耦合深度脱氮的稳定运行。在最佳进水NH-N/NO-N比1:1.1时，进水NH-N和NO-N浓度分别为240 mg·L和265 mg·L，TN去除率达到1.50 kg·(m·d)，厌氧氨氧化和S-SADN途径的TN去除效率分别稳定在(95.68±1.22)%和(2.04±0.77)%。在整个运行过程中，厌氧氨氧化在底物NO-N的竞争中始终占据绝对优势，厌氧氨氧化菌的活性（NH-N/VSS）稳定在(0.166±0.008)kg·(kg·d)。
