Valuyskikh Olga E, Teteryuk Ludmila V, Pylina Yana I, Sushentsov Oleg E, Martynenko Nikita A, Shadrin Dmitry M
Institute of Biology of Komi Scientific Center of Ural Branch of Russian Academy of Sciences, Kommunisticheskaya 28, Syktyvkar, 167982, Russia Komi Scientific Center, Ural Branch of Russian Academy of Sciences Syktyvkar Russia.
Botanical Garden of Ural Branch of Russian Academy of Sciences, 8 Marta 202a, Yekaterinburg, 620144, Russia Botanical Garden, Ural Branch of Russian Academy of Sciences Yekaterinburg Russia.
PhytoKeys. 2020 Oct 9;162:113-130. doi: 10.3897/phytokeys.162.53361. eCollection 2020.
We studied the allopatric complex (L.) Mill. s.lat. (Ranunculaceae) in north-eastern European Russia and the Urals. In this region, there are two kinds of with different perianth colours in monochrome and polychrome populations. To clarify their taxonomic boundaries, we used the sequences of chloroplast DNA (L and K) and nuclear DNA (ITS2), in addition to morphological characteristics. The combination of three markers (L+K+ITS2) was found to be the most effective for phylogenetic resolution. The samples of two morphologically-different taxa and s.str. were shown to form a single clade on the phylogenetic tree. Based on the molecular phylogenetic analysis, we were not able to unequivocally prove the independent existence of .
我们研究了俄罗斯欧洲东北部和乌拉尔地区的广义驴蹄草复合体(毛茛科)。在该地区,单色和多色种群中有两种具有不同花被颜色。为了厘清它们的分类界限,除形态特征外,我们还使用了叶绿体DNA(L和K)和核DNA(ITS2)序列。发现三个标记(L+K+ITS2)的组合对系统发育解析最为有效。两个形态不同的分类群和狭义驴蹄草的样本在系统发育树上显示形成一个单系分支。基于分子系统发育分析,我们无法明确证明[未提及的分类群]的独立存在。