评估血清巨噬细胞移动抑制因子（MIF）、脂联素及其他脂肪因子作为狼疮性肾炎蛋白尿和肾功能不全潜在标志物的研究：一项横断面研究

Assessment of serum macrophage migration inhibitory factor (MIF), adiponectin, and other adipokines as potential markers of proteinuria and renal dysfunction in lupus nephritis: a cross-sectional study.

作者信息

Gamez-Nava Jorge Ivan, Diaz-Rizo Valeria, Perez-Guerrero Edsaul Emilio, Muñoz-Valle Jose Francisco, Saldaña-Cruz Ana Miriam, Fajardo-Robledo Nicte Selene, Jacobo-Cuevas Heriberto, Nava-Valdivia Cesar Arturo, Alcaraz-Lopez Miriam Fabiola, Trujillo Xochitl, Huerta Miguel, Cardona-Muñoz Ernesto German, Gonzalez-Lopez Laura

机构信息

Programa de Doctorado en Farmacología del Departamento de Fisiología y Programa de Doctorado en Salud del Pública Depatamento de Salud Pública, Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Sierra Mojada 950, Colonia Independencia, 44340 Guadalajara, Jalisco Mexico.

Centro Medico Nacional de Occidente, Unidad de Investigacion Biomedica 02, Instituto Mexicano del Seguro Social, Hospital de Especialidades, 44340 Guadalajara, Jalisco Mexico.

出版信息

Biomark Res. 2020 Oct 28;8:55. doi: 10.1186/s40364-020-00236-x. eCollection 2020.

DOI:10.1186/s40364-020-00236-x

PMID:33133605

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7594329/

Abstract

BACKGROUND

To date, the association of serum macrophage migration inhibitory factor (MIF) and serum adipokines with lupus nephritis is controversial.

OBJECTIVE

To assess the utility of serum MIF, leptin, adiponectin and resistin levels as markers of proteinuria and renal dysfunction in lupus nephritis.

METHODS

Cross-sectional study including 196 systemic lupus erythematosus (SLE) patients and 52 healthy controls (HCs). Disease activity was assessed by Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Renal SLE involvement was investigated by renal-SLEDAI. MIF, adiponectin, leptin and resistin levels were quantified by ELISA. We assessed the correlations of quantitative variables by Spearman correlation (r). Multivariable linear regression adjusted the variables associated with the severity of proteinuria.

RESULTS

SLE patients had higher MIF ( = 0.02) and adiponectin ( < 0.001) than HCs. Patients with renal SLE involvement ( = 43) had higher adiponectin (19.0 vs 13.3 μg/mL, = 0.002) and resistin (10.7 vs 8.9 ng/mL, = 0.01) than patients with non-renal SLE ( = 153). Proteinuria correlated with high adiponectin ( = 0.19, < 0.009) and resistin ( = 0.26, < 0.001). MIF ( = 0.27, = 0.04). Resistin correlated with increased creatinine ( = 0.18, = 0.02). High renal-SLEDAI correlated with adiponectin ( = 0.21, = 0.004). Multiple linear regression showed that elevated adiponectin ( = 0.02), younger age ( = 0.04) and low MIF ( = 0.02) were associated with the severity of proteinuria. Low MIF and high adiponectin levels interacted to explain the association with the severity of proteinuria (R = 0.41).

CONCLUSIONS

High adiponectin combined with low MIF concentrations int+eract to explain the severity of proteinuria in renal SLE. These findings highlight the relevance of adiponectin, resistin and MIF as markers of LN.

摘要

背景

迄今为止，血清巨噬细胞移动抑制因子（MIF）和血清脂肪因子与狼疮性肾炎的关联存在争议。

目的

评估血清MIF、瘦素、脂联素和抵抗素水平作为狼疮性肾炎蛋白尿和肾功能不全标志物的效用。

方法

横断面研究纳入196例系统性红斑狼疮（SLE）患者和52例健康对照（HC）。采用系统性红斑狼疮疾病活动指数（SLEDAI）评估疾病活动度。通过肾SLEDAI调查肾脏SLE受累情况。采用酶联免疫吸附测定（ELISA）法对MIF、脂联素、瘦素和抵抗素水平进行定量。我们通过Spearman相关性（r）评估定量变量之间的相关性。多变量线性回归对与蛋白尿严重程度相关的变量进行校正。

结果

SLE患者的MIF（P = 0.02）和脂联素水平（P < 0.001）高于HC。肾脏SLE受累患者（n = 43）的脂联素水平（19.0 vs 13.3μg/mL，P = 0.002）和抵抗素水平（10.7 vs 8.9 ng/mL，P = 0.01）高于非肾脏SLE患者（n = 153）。蛋白尿与高脂联素水平（r = 0.19，P < 0.009）和抵抗素水平（r = 0.26，P < 0.001）相关。MIF也相关（r = 0.27，P = 0.04）。抵抗素与肌酐升高相关（r = 0.18，P = 0.02）。高肾SLEDAI与脂联素相关（r = 0.21，P = 0.004）。多变量线性回归显示，脂联素升高（P = 0.02）、年龄较小（P = 0.04）和低MIF水平（P = 0.02）与蛋白尿严重程度相关。低MIF和高脂联素水平相互作用可解释与蛋白尿严重程度的关联（R = 0.41）。