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同时进行皮层荧光钙成像和全脑 fMRI。

Simultaneous cortex-wide fluorescence Ca imaging and whole-brain fMRI.

机构信息

Department of Radiology and Biomedical Imaging, Yale School of Medicine, New Haven, CT, USA.

Department of Neurobiology, Yale School of Medicine, Yale University, New Haven, CT, United States.

出版信息

Nat Methods. 2020 Dec;17(12):1262-1271. doi: 10.1038/s41592-020-00984-6. Epub 2020 Nov 2.

DOI:10.1038/s41592-020-00984-6
PMID:33139894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7704940/
Abstract

Achieving a comprehensive understanding of brain function requires multiple imaging modalities with complementary strengths. We present an approach for concurrent widefield optical and functional magnetic resonance imaging. By merging these modalities, we can simultaneously acquire whole-brain blood-oxygen-level-dependent (BOLD) and whole-cortex calcium-sensitive fluorescent measures of brain activity. In a transgenic murine model, we show that calcium predicts the BOLD signal, using a model that optimizes a gamma-variant transfer function. We find consistent predictions across the cortex, which are best at low frequency (0.009-0.08 Hz). Furthermore, we show that the relationship between modality connectivity strengths varies by region. Our approach links cell-type-specific optical measurements of activity to the most widely used method for assessing human brain function.

摘要

实现对大脑功能的全面理解需要多种具有互补优势的成像模式。我们提出了一种同时进行宽场光学和功能磁共振成像的方法。通过合并这些模式，我们可以同时获得全脑血氧水平依赖（BOLD）和全皮质钙敏荧光测量的大脑活动。在转基因小鼠模型中，我们使用优化伽马变体传递函数的模型表明，钙可以预测 BOLD 信号。我们发现皮质中存在一致的预测，其在低频（0.009-0.08 Hz）下效果最佳。此外，我们还表明，模态连接强度之间的关系因区域而异。我们的方法将活动的细胞类型特异性光学测量与评估人类大脑功能最广泛使用的方法联系起来。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/949b/7704940/936b7833052b/nihms-1631388-f0006.jpg
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本文引用的文献

[1]
Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits.

Nat Methods. 2019-11-4

[2]
The impact of fasting on resting state brain networks in mice.

Sci Rep. 2019-2-27

[3]
Awake Mouse Imaging: From Two-Photon Microscopy to Blood Oxygen Level-Dependent Functional Magnetic Resonance Imaging.

Biol Psychiatry Cogn Neurosci Neuroimaging. 2018-12-12

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Front Neurosci. 2018-11-2

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Fiber-optic implant for simultaneous fluorescence-based calcium recordings and BOLD fMRI in mice.

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Brain-state dependent astrocytic Ca signals are coupled to both positive and negative BOLD-fMRI signals.

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Resting-state functional MRI reveals altered brain connectivity and its correlation with motor dysfunction in a mouse model of Huntington's disease.

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