在用于区分三氯蔗糖的自动化微流控电子舌测试中,流速的影响。

Influence of the Flow Rate in an Automated Microfluidic Electronic Tongue Tested for Sucralose Differentiation.

机构信息

Department of Applied Physics, "Gleb Wataghin" Institute of Physics (IFGW), University of Campinas (UNICAMP), Campinas SP 13083-859, Brazil.

Quantum Design Latin America, Campinas SP 13080-655, Brazil.

出版信息

Sensors (Basel). 2020 Oct 30;20(21):6194. doi: 10.3390/s20216194.

Abstract

Incorporating electronic tongues into microfluidic devices brings benefits as dealing with small amounts of sample/discharge. Nonetheless, such measurements may be time-consuming in some applications once they require several operational steps. Here, we designed four collinear electrodes on a single printed circuit board, further comprised inside a straight microchannel, culminating in a robust e-tongue device for faster data acquisition. An analog multiplexing circuit automated the signal's routing from each of the four sensing units to an impedance analyzer. Both instruments and a syringe pump are controlled by dedicated software. The automated e-tongue was tested with four Brazilian brands of liquid sucralose-based sweeteners under 20 different flow rates, aiming to systematically evaluate the influence of the flow rate in the discrimination among sweet tastes sold as the same food product. All four brands were successfully distinguished using principal component analysis of the raw data, and despite the nearly identical sucralose-based taste in all samples, all brands' significant distinction is attributed to small differences in the ingredients and manufacturing processes to deliver the final food product. The increasing flow rate improves the analyte's discrimination, as the silhouette coefficient reaches a plateau at ~3 mL/h. We used an equivalent circuit model to evaluate the raw data, finding a decrease in the double-layer capacitance proportional to improvements in the samples' discrimination. In other words, the flow rate increase mitigates the formation of the double-layer, resulting in faster stabilization and better repeatability in the sensor response.

摘要

将电子舌整合到微流控设备中具有处理少量样品/排放物的优势。然而,在某些应用中,由于需要多个操作步骤,这种测量可能会很耗时。在这里,我们在单个印刷电路板上设计了四个共线电极,进一步包含在直微通道内,最终形成了一个稳健的电子舌设备,用于更快的数据采集。模拟多路复用电路自动将来自四个传感单元的信号路由到阻抗分析仪。仪器和注射器泵都由专用软件控制。自动化电子舌在 20 种不同流速下对四种巴西品牌的液体三氯蔗糖甜味剂进行了测试,旨在系统评估流速对作为相同食品销售的甜味剂的鉴别影响。使用原始数据的主成分分析成功区分了所有四个品牌,尽管所有样品中的三氯蔗糖味道几乎相同,但所有品牌的显著区别归因于成分和制造工艺的微小差异,以提供最终的食品产品。随着流速的增加,分析物的分辨能力得到提高,因为轮廓系数在~3mL/h 时达到一个平台。我们使用等效电路模型来评估原始数据,发现双电层电容随样品分辨能力的提高而呈比例下降。换句话说,流速的增加减轻了双电层的形成,从而在传感器响应中更快地稳定并提高重复性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6902/7662545/0aa658334749/sensors-20-06194-g001.jpg

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