Jeong Heon-Ho
Department of Chemical and Biomolecular Engineering, Chonnam National University, Yeosu, Jeonnam, Republic of Korea.
SLAS Technol. 2021 Apr;26(2):159-164. doi: 10.1177/2472630320969146. Epub 2020 Nov 4.
Bacterial motility in response to chemicals, also called bacterial chemotaxis, is a critical ability to search for the optimal environment to ensure the survival of bacterial species. Recent advances in microbiology have allowed the engineering of bacterial chemotactic properties. Conventional methods for characterizing bacterial motility are not able to fully monitor chemotactic behavior. Developments in microfluidic technology have enabled the designing of new experimental protocols in which spatiotemporal control of the cellular microenvironment can be achieved, and in which bacterial motility can be precisely and quantitatively measured and compared. This review provides an overview of recent developments of and new insights into microfluidic systems for chemotaxis assay.
细菌对化学物质的运动反应，也称为细菌趋化性，是寻找最佳环境以确保细菌物种生存的关键能力。微生物学的最新进展使得对细菌趋化特性进行工程改造成为可能。传统的表征细菌运动性的方法无法完全监测趋化行为。微流控技术的发展使得设计新的实验方案成为可能，在这些方案中可以实现对细胞微环境的时空控制，并且可以精确和定量地测量及比较细菌的运动性。本综述概述了用于趋化性测定的微流控系统的最新进展和新见解。
