Hoplite Lab, Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada.
J Fish Dis. 2021 Jun;44(6):757-769. doi: 10.1111/jfd.13299. Epub 2020 Nov 4.
Hydrogen peroxide (H O ) is used to treat sea lice infections of farmed salmonids in the Atlantic and Pacific Oceans and issues with resistance to this treatment, and others are a major threat to the sustainability of the industry. The objectives of this study were to determine how H O exposure affects survival and antioxidant-related gene expression in salmon lice (Lepeophtheirus salmonis) collected from the Bay of Fundy, New Brunswick. The maximum recommended dose of H O is 1,800 mg/L, while the EC values (with 95% CI) for the population tested were 1,486 (457, 2,515) mg/L for males and 2,126 (984, 3,268) mg/L for females. Neither temperature nor pretreatment with emamectin benzoate (EMB) impacted survival after H O exposure. RT-qPCR was performed on pre-adult sea lice exposed to H O and showed that four genes classically involved in the response to oxidative stress were unchanged between treated and control groups. Seven genes were found to be significantly upregulated in males and one in females. This is the first report on the efficacy and molecular responses of Atlantic Canada sea lice to H O treatment.
过氧化氢（H₂O₂）用于治疗大西洋和太平洋养殖鲑鱼的海虱感染以及对这种治疗方法产生抗药性的问题，这些问题对该行业的可持续性构成了重大威胁。本研究的目的是确定从新不伦瑞克省芬迪湾采集的鲑鱼虱（Lepeophtheirus salmonis）暴露于 H₂O₂后对其生存和抗氧化相关基因表达的影响。H₂O₂的最大推荐剂量为 1800 mg/L，而经测试的种群的 EC 值（95%CI）为雄性 1486（457，2515）mg/L，雌性 2126（984，3268）mg/L。H₂O₂暴露后，温度或提前用埃玛菌素苯甲酸盐（EMB）预处理均未影响生存。对暴露于 H₂O₂的幼体海虱进行 RT-qPCR 检测，结果表明，在处理组和对照组之间，经典参与氧化应激反应的四个基因没有变化。在雄性中发现七个基因显著上调，在雌性中发现一个基因显著上调。这是关于加拿大大西洋地区海虱对 H₂O₂处理的功效和分子反应的首次报道。
