溶瘤呼肠孤病毒对脐带间充质干细胞生物学活性的影响。
The influence of oncolytic reovirus on the biological activities of umbilical cord-derived mesenchymal stem cells.
机构信息
Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Oct;24(20):10839-10849. doi: 10.26355/eurrev_202010_23446.
OBJECTIVE
To investigate the influence of oncolytic reovirus on the biological activities of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) as a novel virotherapy strategy.
MATERIALS AND METHODS
The Cell Counting Kit-8 assay was used to detect the viability of hUC-MSCs infected with different multiplicities of infection (MOIs) of reoviruses. The biological activities (proliferation, marker expression, multipotency, and migration) of hUC-MSCs were verified by assaying osteogenic and adipogenic differentiation potential, flow cytometry, and electrical cell-substrate impedance sensing, respectively.
RESULTS
The viability of hUC-MSCs slightly decreased by infection with low titers of reoviruses. A MOI of 1 had no effect on the viability of hUC-MSCs within 96 h. The biological activities (proliferation, marker expression, multipotency, and migration) of hUC-MSCs were not affected by reovirus infection at a MOI of 1.
CONCLUSIONS
Reovirus at a MOI of 1 had no effect on the biological activities of hUC-MSCs.
目的
研究溶瘤呼肠孤病毒对人脐带间充质干细胞（hUC-MSCs）生物学活性的影响，探索其作为一种新型病毒疗法的策略。
材料与方法
采用细胞计数试剂盒-8 法检测不同感染复数（MOI）的呼肠孤病毒感染 hUC-MSCs 后的细胞活力。通过检测成骨和脂肪分化潜能、流式细胞术和电细胞-底物阻抗传感分别验证 hUC-MSCs 的生物学活性（增殖、标志物表达、多能性和迁移）。
结果
低滴度呼肠孤病毒感染可使 hUC-MSCs 的活力略有下降。在 96 h 内，MOI 为 1 对 hUC-MSCs 的活力无影响。MOI 为 1 时，呼肠孤病毒感染对 hUC-MSCs 的生物学活性（增殖、标志物表达、多能性和迁移）没有影响。
结论
MOI 为 1 的呼肠孤病毒对 hUC-MSCs 的生物学活性没有影响。
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