School of Chemical and Biomolecular Engineering, Pusan National University, Busan 46241, Republic of Korea.
Gwangju Bioenergy R&D Center, Korea Institute of Energy Research (KIER), Gwangju, Republic of Korea.
Bioresour Technol. 2021 Jan;320(Pt A):124333. doi: 10.1016/j.biortech.2020.124333. Epub 2020 Oct 29.
Conventional photoheterotrophic H production by purple sulfur bacteria requires additional organic substrates as the carbon and energy sources. This study examined the novel photoautotrophic H production of Rhodobacter sphaeroides with concomitant CO uptake in microbial electrosynthesis (MES). Under an applied potential of -0.9 V vs. Ag/AgCl to the cathode, Rhodobacter sphaeroides produced hydrogen with CO as the sole carbon source under illumination. The initial planktonic cells decreased rapidly in suspension, whereas biomass formation on the cathode surface increased gradually during MES operation. The electron and carbon flow under photoautotrophic conditions in MES were estimated. Glutamate, as the nitrogen source, enhanced hydrogen production significantly (328 mL/L/day) compared to NHCl (67 mL/L/day) during seven days of operation. The photoautotrophic condition with 6000 lx presented CO consumption and simultaneous biomass formation on the cathode electrode. MES-driven electron and proton transfer enabled the simultaneous production of hydrogen and CO uptake
传统的紫色硫细菌异养产氢需要额外的有机底物作为碳源和能源。本研究考察了在微生物电解池（MES）中，球形红杆菌的新型光自养产氢和同时 CO 摄取。在施加到阴极的-0.9 V vs. Ag/AgCl 电位下，在光照下，球形红杆菌以 CO 为唯一碳源产氢。初始浮游细胞在悬浮液中迅速减少，而在 MES 运行过程中，在阴极表面形成生物量逐渐增加。在 MES 中的光自养条件下估算了电子和碳流。与 NHCl（67 mL/L/天）相比，在 7 天的运行中，作为氮源的谷氨酸显著提高了产氢量（328 mL/L/天）。在 6000 lx 的光自养条件下，阴极电极上同时进行 CO 消耗和生物量形成。MES 驱动的电子和质子转移使同时生产氢气和 CO 摄取成为可能。
