一例罕见的天然产生的抗Jk(a)抗体在试管法手工筛查试验中漏检的病例。
A rare case of naturally occurring allo anti-Jk(a) missed in manual screening test on tube method.
作者信息
Kanani Ashish N, Senjaliya Snehal B, Rajapara Manisha M, Gupta Anurag K, Joshi Sanmukh R
机构信息
Lok Samarpan Regional Blood Center, Surat, Gujarat, India.
Department of Application, Immucor (India), New Delhi, India.
出版信息
Asian J Transfus Sci. 2020 Jan-Jun;14(1):60-62. doi: 10.4103/ajts.AJTS_104_19. Epub 2020 Jul 24.
BACKGROUND
Antibodies to the Kidd blood group are mainly red blood cell (RBC) immune, but a few reports on naturally occurring antibodies have been documented.
AIM
The aim of this study is to study the anti-Jk(a) for its unusual reactivity with different serological methods.
MATERIALS AND METHODS
Donor's plasma was tested with RBCs from in house donors and commercial panels by manual and automated devices.
RESULTS
A 36-year-old male blood donor with naturally occurring anti-Jk(a) is detected by solid-phase assays and the gel card technique but not by the tube method. The IgG antibody with the titer of >32 was not a complement-fixing hemolysin, showed a reduced reactivity with enzyme-treated RBCs, and was detectable through 8 months' follow-up period. The donor was typed as (Jk(a-).
CONCLUSION
An unusual naturally occurring anti-Jk(a) detected by solid-phase red-cell adherence but not reacting by tube technique reflected on the sensitivity of the methods used.
背景
抗基德血型抗体主要是红细胞免疫性的，但已有少数关于天然存在抗体的报道。
目的
本研究旨在研究抗-Jk(a)抗体在不同血清学方法下的异常反应性。
材料与方法
用手工和自动化设备，将供者血浆与来自内部供者的红细胞及商业红细胞面板进行检测。
结果
通过固相检测法和凝胶卡技术检测到一名36岁男性献血者天然存在抗-Jk(a)抗体，但试管法未检测到。效价>32的IgG抗体不是补体结合溶血素，与酶处理的红细胞反应性降低，且在8个月的随访期内均可检测到。该供者血型为Jk(a-)。
结论
通过固相红细胞黏附法检测到的一种异常天然抗-Jk(a)抗体，而试管法未检测到，这反映了所用方法的敏感性。
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