Liu Meimei, Hu Ruqian, Guo Yaowen, Sun Weizhong, Li Jiantao, Fan Mingliang, Wang Yan, Du Huamao, Tang Zhiru, Chai Chunli
College of Biotechnology, Southwest University, Chongqing 400715, China.
College of Animal Science, Southwest University, Chongqing 400715, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Sep 25;36(9):1887-1898. doi: 10.13345/j.cjb.200024.
We studied the influence of Lactobacillus reuteri SL001 (L. reuteri SL001) on the gut microbial community in Alzheimer's disease model mice (APP/PS1 double transgenic mice, ADmice) and wild type (C57BL/6) mice. The AD model mice and wild type mice were divided into test and control groups (4 in total), with 5 mice each and only male mice. The test group was fed with 0.2 mL suspension of L. reuteri SL001 at 5×10¹¹ CFU/mL. The control group received the same amount of sterile PBS daily for 45 days. Fecal samples were collected to compare and analyze the community structure and diversity of microbiota through high-throughput sequencing of the V3-V4 region of 16S rRNA gene. By sequence alignment and classification, the intestinal microbial OTUs of the 4 groups including 19 phyla, 40 classes, 64 orders, 104 families and 175 genera. The α diversity of AD model mice was greater than that of wild type mice, but the difference between the two was small. After adding L. reuteri SL001, the α diversity of both mice increased, and the increase in AD model mice was smaller. At the phyla level, the dominant phyla of the four groups of mice were Bacteroidetes, Firmicutes and Proteobacteria. The abundance of Bacteroides phylum in AD model mice was lower than that of wild type, and the abundance of chlamydomonas was higher than that of wild type. Feeding L. reuteri SL001 reduced the proportion of Firmicutes/Bacteroidetes (F/B) in mice. At the order level, the relative abundance of Bacteroidales, Lactobacillales, Bacillales and Bifidobacteriales in AD model mice was lower than that of wild type mice. At the genus level, the abundant genera were Allobaculum, Lachnospiraceae_NK4A136_group, Bacteroides and Lactobacillus. The relative abundance of Bacteroides, Lactobacillus, Bifidobacterium and Alloprevotella in AD model mice was lower than that in wild type mice. Adding L. reuteri SL001 increased the abundance of these genera and Bacteroides, Lactobacillus, Bacillus and Bifidobacteria in AD model mice. The relative abundance of Butyrivibrio in AD model mice was also lower than that in wild type, but after the feeding of L. reuteri SL001, the relative abundance was reduced in both mice. The dominant strains of wild-type mice were Lactobacillus, and no dominant flora was found in AD model mice. The results in this article provide valuable data for revealing the difference in intestinal microbial diversity between AD model mice and C57BL/6 mice, and feeding L. reuteri SL001 play positive roles in adjusting the intestinal bacterial community structure of AD model mice.
我们研究了罗伊氏乳杆菌SL001（L. reuteri SL001）对阿尔茨海默病模型小鼠（APP/PS1双转基因小鼠，AD小鼠）和野生型（C57BL/6）小鼠肠道微生物群落的影响。将AD模型小鼠和野生型小鼠分为试验组和对照组（共4组），每组5只且均为雄性小鼠。试验组小鼠每日灌胃0.2 mL浓度为5×10¹¹ CFU/mL的罗伊氏乳杆菌SL001悬液。对照组每天接受等量的无菌PBS，持续45天。收集粪便样本，通过对16S rRNA基因V3-V4区域进行高通量测序，比较和分析微生物群的群落结构和多样性。通过序列比对和分类，4组小鼠的肠道微生物OTU包括19个门、40个纲、64个目、104个科和175个属。AD模型小鼠的α多样性大于野生型小鼠，但两者差异较小。添加罗伊氏乳杆菌SL001后，两种小鼠的α多样性均增加，且AD模型小鼠的增加幅度较小。在门水平上，4组小鼠的优势菌门为拟杆菌门、厚壁菌门和变形菌门。AD模型小鼠中拟杆菌门的丰度低于野生型，衣原体的丰度高于野生型。饲喂罗伊氏乳杆菌SL001降低了小鼠中厚壁菌门/拟杆菌门（F/B）的比例。在目水平上，AD模型小鼠中拟杆菌目、乳杆菌目、芽孢杆菌目和双歧杆菌目的相对丰度低于野生型小鼠。在属水平上，优势属为别氏菌属、毛螺菌科_NK4A136_组、拟杆菌属和乳杆菌属。AD模型小鼠中拟杆菌属、乳杆菌属、双歧杆菌属和异普雷沃菌属的相对丰度低于野生型小鼠。添加罗伊氏乳杆菌SL001增加了AD模型小鼠中这些属以及拟杆菌属、乳杆菌属、芽孢杆菌属和双歧杆菌属的丰度。AD模型小鼠中丁酸弧菌属的相对丰度也低于野生型，但饲喂罗伊氏乳杆菌SL001后，两种小鼠中的相对丰度均降低。野生型小鼠的优势菌株为乳杆菌属，AD模型小鼠中未发现优势菌群。本文的研究结果为揭示AD模型小鼠与C57BL/6小鼠肠道微生物多样性差异提供了有价值的数据，且饲喂罗伊氏乳杆菌SL001对调节AD模型小鼠肠道细菌群落结构具有积极作用。
